Aubourg S, Takvorian A, Chéron A, Kreis M, Lecharny A
Laboratoire de Biologie du Développement des Plantes, Institut de Biotechnologie des Plantes, ERS/CNRS 569, Université de Paris-Sud, Orsay, France.
Gene. 1997 Oct 15;199(1-2):241-53. doi: 10.1016/s0378-1119(97)00374-0.
In the framework of the complete genome sequencing programme of the crucifer Arabidopsis thaliana, a 23.9-kb fragment from the long arm of chromosome IV has been analysed. This paper presents a methodological approach, integrating computerized predictions, database screening, the sequencing of cognate cDNAs and a PCR-based detection of expression that allows the accumulation of an important amount of information from an anonymous sequence. This work revealed the organization of novel genes and the vestige of a copia-like retrotransposon. The gene AtRH1 encodes the first member of a new subfamily of the plant DEAD box RNA helicases. A recurrent and complete search of dbEST has been used to evaluate the number of different RNA helicases expressed in A. thaliana. On the 18 discriminated members of the family, only a small number seems to be expressed at a relatively high level. The putative gene AtTS1 encodes a novel terpene synthase in A. thaliana, and the genes G14587-5 and G14587-6 encode unknown proteins. This study illustrates most of the situations that could be encountered during the analysis of an anonymous sequence from A. thaliana.
在十字花科植物拟南芥全基因组测序计划的框架下，对来自第四条染色体长臂的一个23.9千碱基对的片段进行了分析。本文介绍了一种方法，该方法整合了计算机预测、数据库筛选、同源cDNA测序以及基于PCR的表达检测，从而能够从一个匿名序列中积累大量信息。这项工作揭示了新基因的组织情况以及一个类copia反转录转座子的遗迹。基因AtRH1编码植物DEAD盒RNA解旋酶一个新亚家族的首个成员。通过对dbEST进行反复全面搜索，评估了拟南芥中表达的不同RNA解旋酶的数量。在该家族18个已区分的成员中，只有少数似乎以相对较高的水平表达。推定基因AtTS1在拟南芥中编码一种新的萜类合酶，基因G14587 - 5和G14587 - 6编码未知蛋白质。这项研究说明了在分析拟南芥的匿名序列时可能遇到的大多数情况。
