Bailey A L, Wortley G, Southon S
Nutrition, Diet and Health Department, Institute of Food Research, UK.
Free Radic Biol Med. 1997;23(7):1078-85. doi: 10.1016/s0891-5849(97)00162-7.
A modified procedure is presented for the HPLC determination of nanomolar concentrations of n-alkanals, hydroxyalkenals, malondialdehyde and furfural in biological fluid. The modifications allow aldehyde profile analysis of small samples of fresh, human, low density lipoprotein (LDL), enabling more detailed studies of LDL fatty acid peroxidation. Aldehydes are reacted with 1,3-cyclohexanedione to produce fluorescent derivatives which are separated by gradient, reversed phase, high performance liquid chromatography (HPLC). Analysis time has been reduced by shortening the sample preparation. Sensitivity has been increased by miniaturization of the derivatisation procedure, reducing required sample size. Recoveries of added aldehydes have been improved. In addition, the method presented allows determination of three further aldehydes, not measured previously by CHD methods: malondialdehyde, formaldehyde and furfural. Recovery and variability data and concentrations of aldehydes found in human LDL are given. The capacity of the method for further development, to enable determination of other aldehydes such as the trans, 2-alkenals, is also demonstrated.
本文介绍了一种改进的高效液相色谱法，用于测定生物流体中纳摩尔浓度的正构烷醛、羟基烯醛、丙二醛和糠醛。这些改进使得能够对新鲜的人低密度脂蛋白（LDL）小样本进行醛类分析，从而更详细地研究LDL脂肪酸过氧化。醛类与1,3 - 环己二酮反应生成荧光衍生物，通过梯度反相高效液相色谱（HPLC）进行分离。通过缩短样品制备时间，分析时间得以减少。通过衍生化程序的小型化提高了灵敏度，减少了所需样品量。添加醛类的回收率得到了提高。此外，本文提出的方法能够测定另外三种以前冠心病方法未测定的醛类：丙二醛、甲醛和糠醛。给出了人LDL中醛类的回收率、变异性数据及浓度。还展示了该方法进一步发展以测定其他醛类（如反式2 - 烯醛）的能力。
