van Kuijk F J, Siakotos A N, Fong L G, Stephens R J, Thomas D W
Department of Chemistry and Biochemistry, Montana State University, Bozeman 59717.
Anal Biochem. 1995 Jan 1;224(1):420-4. doi: 10.1006/abio.1995.1060.
Previously, we reported on the determination of 4-hydroxyalkenals as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry. In this study, an improved method is presented which allows quantitative detection of 4-hydroxyalkenals in tissues by using two stable isotope-labeled internal standards, 9D3-4-hydroxynonenal and 9D3-4-hydroxyhexenal. This assay was used to quantitate 4-hydroxyalkenals in copper-oxidized human low-density lipoprotein, and we found more 4-hydroxynonenal than 4-hydroxyhexenal. This is consistent with the fact that there are more omega-6 than omega-3 fatty acids in human low-density lipoprotein samples, which are the sources of 4-hydroxynonenal and 4-hydroxyhexenal, respectively.
此前，我们报道了通过气相色谱-质谱法将4-羟基烯醛测定为五氟苄基肟衍生物。在本研究中，提出了一种改进方法，该方法允许使用两种稳定同位素标记的内标物9D3-4-羟基壬烯醛和9D3-4-羟基己烯醛对组织中的4-羟基烯醛进行定量检测。该测定法用于定量铜氧化的人低密度脂蛋白中的4-羟基烯醛，我们发现4-羟基壬烯醛比4-羟基己烯醛更多。这与人类低密度脂蛋白样品中ω-6脂肪酸比ω-3脂肪酸更多这一事实一致，它们分别是4-羟基壬烯醛和4-羟基己烯醛的来源。
