Masuzawa T, Suzuki H, Kawabata H, Ishiguro F, Takada N, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Wildl Dis. 1996 Oct;32(4):565-71. doi: 10.7589/0090-3558-32.4.565.
Spirochetes were isolated from the tick, Ixodes tanuki, as well as wood mice (Apodemus speciosus) and voles (Clethrionomys rufocanus and Eothenomys smithii), caught in Fukui, Tokushima, and Hokkaido, Japan, from 1991 to 1993. Spirochetes were characterized on the basis of protein profiles, reactivities with monoclonal antibodies (mAbs), Outer surface protein A gene (ospA) and Outer surface protein B gene (ospB) amplification analysis, rRNA gene and flagellin gene restriction fragment length polymorphism (RFLP) analysis, and DNA homology values. Protein profiles of all isolates were homologous and reacted with mAb to OspA, OspB, OspC, flagellin, and heat shock protein 60. The primer reactivity to ospA and ospB were different from those of Borrelia burgdorferi sensu stricto, B. afzelii, B. japonica, and B. garinii. Based on the DNA/DNA homology value and RFLP analysis of rRNA and flagellin gene, these Borrelia sp. isolates are a new group of B. burgdorferi sensu lato. The isolates from ticks and the host rodents were identical in these assays. We propose that these Borrelia sp. are adapted to I. tanuki and are maintained in these field rodents.
1991年至1993年期间，在日本福井、德岛和北海道捕获的蜱（日本伊蚊蜱）以及木鼠（日本姬鼠）和田鼠（棕背䶄和斯氏高山䶄）中分离出了螺旋体。根据蛋白质谱、与单克隆抗体（mAb）的反应性、外表面蛋白A基因（ospA）和外表面蛋白B基因（ospB）扩增分析、rRNA基因和鞭毛蛋白基因限制性片段长度多态性（RFLP）分析以及DNA同源性值对螺旋体进行了鉴定。所有分离株的蛋白质谱均具有同源性，并与针对OspA、OspB、OspC、鞭毛蛋白和热休克蛋白60的单克隆抗体发生反应。对ospA和ospB的引物反应性与狭义伯氏疏螺旋体、阿氏疏螺旋体、日本疏螺旋体和伽氏疏螺旋体不同。基于rRNA和鞭毛蛋白基因的DNA/DNA同源性值和RFLP分析，这些疏螺旋体分离株是伯氏疏螺旋体广义种的一个新组。在这些检测中，蜱和宿主啮齿动物的分离株是相同的。我们认为这些疏螺旋体适应了日本伊蚊蜱，并在这些野外啮齿动物中得以维持。
