Masuzawa T, Suzuki H, Kawabata H, Ishiguro F, Takada N, Yano Y, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Clin Microbiol. 1995 May;33(5):1392-4. doi: 10.1128/jcm.33.5.1392-1394.1995.
Spirochetes isolated from three species of wild mice, Apodemus speciosus, Apodemus argenteus, and Eothenomys smithi, which were caught on Honshu, Japan, were characterized on the bases of protein profiles, reactivities with monoclonal antibodies (MAbs), and DNA homology values. All the isolates reacted with MAb O1441b, which is specific for Borrelia japonica, but not with MAb P62, which is specific for Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii. The DNA relatedness of four representatives isolates to strain HO14, a type strain of B. japonica, was 83 to 88%. From these findings, the spirochetes were identified as B. japonica.
从日本本州捕获的三种野生小鼠，即大林姬鼠、银色姬鼠和中华姬鼠中分离出的螺旋体，根据蛋白质谱、与单克隆抗体（MAb）的反应性以及DNA同源性值进行了特征鉴定。所有分离株均与针对日本疏螺旋体的单克隆抗体O1441b发生反应，但不与针对狭义伯氏疏螺旋体、伽氏疏螺旋体和阿氏疏螺旋体的单克隆抗体P62发生反应。四个代表性分离株与日本疏螺旋体模式菌株HO14的DNA相关性为83%至88%。根据这些发现，这些螺旋体被鉴定为日本疏螺旋体。
