Samore M, Killgore G, Johnson S, Goodman R, Shim J, Venkataraman L, Sambol S, DeGirolami P, Tenover F, Arbeit R, Gerding D
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
J Infect Dis. 1997 Nov;176(5):1233-8. doi: 10.1086/514117.
In a collaborative study by three laboratories, arbitrarily primed polymerase chain reaction (AP-PCR), HindIII restriction enzyme analysis (REA), and pulsed-field gel electrophoresis (PFGE) using SmaI were compared for typing of Clostridium difficile. The study included 30 isolates from nosocomial outbreaks in six geographically disparate hospitals and 15 isolates from sporadic cases of C. difficile diarrhea. REA distinguished a total of 23 types representing 10 groups; AP-PCR performed at Deaconess Hospital resolved 19 types; AP-PCR performed at the Centers for Disease Control resolved 15 types. Thirty isolates exhibited degradation of larger sized fragments during processing and therefore were nontypeable by PFGE; among the remaining 15 isolates, PFGE resolved 11 types. Outbreak isolates in five different hospitals represented REA group J and constituted a single AP-PCR strain. In summary, nosocomial outbreaks of C. difficile diarrhea in five hospitals were associated with a single genetic lineage as resolved by multiple strain typing systems.
在三个实验室的一项合作研究中，对用于艰难梭菌分型的任意引物聚合酶链反应（AP-PCR）、HindIII限制性内切酶分析（REA）以及使用SmaI的脉冲场凝胶电泳（PFGE）进行了比较。该研究包括来自六家地理位置分散的医院医院感染暴发的30株分离株以及来自艰难梭菌腹泻散发病例的15株分离株。REA共区分出代表10个组的23种类型；在迪肯尼斯医院进行的AP-PCR分辨出19种类型；在疾病控制中心进行的AP-PCR分辨出15种类型。30株分离株在处理过程中表现出较大片段的降解，因此无法通过PFGE分型；在其余15株分离株中，PFGE分辨出11种类型。五家不同医院的暴发分离株代表REA J组，并构成单一的AP-PCR菌株。总之，通过多种菌株分型系统解析，五家医院的艰难梭菌腹泻医院感染暴发与单一遗传谱系相关。
