哺乳动物肌动蛋白相关蛋白2/3复合物定位于片状伪足突出区域，由进化上保守的蛋白质组成。
Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins.
作者信息
Machesky L M, Reeves E, Wientjes F, Mattheyse F J, Grogan A, Totty N F, Burlingame A L, Hsuan J J, Segal A W
机构信息
Department of Medicine, University College London, U.K.
出版信息
Biochem J. 1997 Nov 15;328 ( Pt 1)(Pt 1):105-12. doi: 10.1042/bj3280105.
Abstract
Human neutrophils contain a complex of proteins similar to the actin-related protein 2/3 (Arp2/3) complex of Acanthamoeba. We have obtained peptide sequence information for each member of the putative seven-protein complex previously described for Acanthamoeba and human platelets. From the peptide sequences we have identified cDNA species encoding three novel proteins in this complex. We find that in addition to Arp2 and Arp3, this complex contains a relative of the human (Suppressor of Profilin) SOP2Hs protein and four previously unknown proteins. These proteins localize in the cytoplasm of fibroblasts that lack lamellipodia, but are enriched in lamellipodia on stimulation with serum or platelet-derived growth factor. We propose a conserved and dynamic role for this complex in the organization of the actin cytoskeleton.
摘要
人类中性粒细胞含有一种与棘阿米巴的肌动蛋白相关蛋白2/3（Arp2/3）复合物相似的蛋白质复合物。我们已经获得了先前描述的棘阿米巴和人类血小板中假定的七蛋白复合物每个成员的肽序列信息。从这些肽序列中，我们鉴定出了编码该复合物中三种新蛋白质的cDNA种类。我们发现，除了Arp2和Arp3外，该复合物还包含人类（抑制蛋白）SOP2Hs蛋白的一个亲属以及四种以前未知的蛋白质。这些蛋白质定位于缺乏片状伪足的成纤维细胞的细胞质中，但在用血清或血小板衍生生长因子刺激后在片状伪足中富集。我们提出该复合物在肌动蛋白细胞骨架的组织中具有保守和动态的作用。
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本文引用的文献
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The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches.包含肌动蛋白相关蛋白Arp2和Arp3的复合体是酵母肌动蛋白斑的运动性和完整性所必需的。
Curr Biol. 1997 Jul 1;7(7):519-29. doi: 10.1016/s0960-9822(06)00223-5.
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J Cell Biol. 1997 Jan 27;136(2):331-43. doi: 10.1083/jcb.136.2.331.
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