Tsuruoka H, Xu H, Kuroda K, Hosaka Y
Department of Virology and Immunology, Osaka University of Pharmaceutical Sciences.
Nihon Rinsho. 1997 Oct;55(10):2714-8.
Gastroenteritis, arthralgia and myalgia are frequently associated with influenza virus infections in humans. One explanation for these symptoms may be that they are due to extra respiratory transmission of virus by viremia. We tried to detect genomic viral RNA of the nucleoprotein (NP) and H3 subtype hemagglutinin (HA) genes by method of RT-PCR in peripheral blood mononuclear cells (PBMC) of 18 children aged 1-14 who suffered from an influenza outbreak in the Kansai district of Japan between December 1992 and February 1993. Three of the 18 samples were RT-PCR positive. The NP gene sequence observed in one patient's PBMC was identical to that obtained from his throat swab fluid. The HA gene sequences observed in the two other PBMC differed from those of RT-PCR amplified DNA from throat swabs by an order of 3-9 nucleotides. Moreover we tried to isolate virus by co-culture with MDCK cells and RBC or WBC of the patients from an influenza outbreak between December 1993 and March 1994. No virus was isolated from 9 patients suffering from H3 subtype but virus was isolated from 5 of 17 patients suffering from type B influenza virus. We believe these results suggest that the viremia on influenza infection is not so rare.
肠胃炎、关节痛和肌痛常与人类流感病毒感染相关。对这些症状的一种解释可能是，它们是由病毒血症导致的病毒呼吸道外传播引起的。我们尝试通过逆转录聚合酶链反应（RT-PCR）方法，在1992年12月至1993年2月间日本关西地区爆发流感时患病的18名1 - 14岁儿童的外周血单核细胞（PBMC）中，检测核蛋白（NP）基因和H3亚型血凝素（HA）基因的基因组病毒RNA。18个样本中有3个RT-PCR呈阳性。在一名患者的PBMC中观察到的NP基因序列与从其咽拭子样本中获得的序列相同。在另外两名患者的PBMC中观察到的HA基因序列，与从咽拭子中RT-PCR扩增的DNA序列相比，有3 - 9个核苷酸的差异。此外，我们尝试通过与MDCK细胞以及1993年12月至1994年3月间流感爆发患者的红细胞或白细胞共培养来分离病毒。9名感染H3亚型的患者中未分离到病毒，但17名感染B型流感病毒的患者中有5名分离到了病毒。我们认为这些结果表明，流感感染时的病毒血症并非罕见。
