Montoro A, Perteguer M J, Chivato T, Laguna R, Cuéllar C
Servicio de Inmunología y Alergologia, Hospital del Aire, Madrid, Spain.
Allergy. 1997 Oct;52(10):985-91. doi: 10.1111/j.1398-9995.1997.tb02418.x.
This study aimed to determine the cause of acute recidivous urticaria in patients who usually eat fish or other seafood. Twenty-five patients were studied. The skin prick test with larval Anisakis simplex extract was performed; total and specific IgE against A. simplex was measured with the CAP System; specific antibodies to A. simplex were determined by ELISA; and immunorecognition patterns of the sera were studied by Western blot. Nineteen patients showed specific IgE to A. simplex, but specific IgE to Ascaris was demonstrated in only two patients. No patients reacted to Toxocara canis or Echinoccocus granulosus antigens with the same test. The skin prick test was positive in 16 patients, in two of them persisting for 48 h. Five patients showed neither skin reaction nor specific IgE to A. simplex. Sera showed specific immunoglobulin levels against A. simplex larval crude extract, by both ELISA and Western blot. Likewise, specific immunoglobulin levels against excretory-secretory antigen were also measured by ELISA. Only one patient showed sensitization to fish. A. simplex was found to be the main cause of acute recidivous urticaria in patients who usually eat fish and are not sensitized to it.
本研究旨在确定经常食用鱼类或其他海鲜的患者急性复发性荨麻疹的病因。对25名患者进行了研究。用简单异尖线虫幼虫提取物进行皮肤点刺试验；采用CAP系统检测针对简单异尖线虫的总IgE和特异性IgE；通过酶联免疫吸附测定法（ELISA）测定针对简单异尖线虫的特异性抗体；并通过蛋白质印迹法研究血清的免疫识别模式。19名患者显示对简单异尖线虫有特异性IgE，但仅两名患者显示对蛔虫有特异性IgE。在相同检测中，没有患者对犬弓首蛔虫或细粒棘球绦虫抗原产生反应。16名患者的皮肤点刺试验呈阳性，其中两名患者的阳性反应持续48小时。五名患者对简单异尖线虫既无皮肤反应也无特异性IgE。通过ELISA和蛋白质印迹法，血清显示出针对简单异尖线虫幼虫粗提物的特异性免疫球蛋白水平。同样，也通过ELISA测定了针对排泄-分泌抗原的特异性免疫球蛋白水平。只有一名患者对鱼类敏感。结果发现，简单异尖线虫是经常食用鱼类且对其不敏感的患者急性复发性荨麻疹的主要病因。
