Pascual C Y, Crespo J F, San Martin S, Ornia N, Ortega N, Caballero T, Muñoz-Pereira M, Martin-Esteban M
Hospital Universitario LA PAZ, Madrid, Spain.
Allergy. 1997 May;52(5):514-20. doi: 10.1111/j.1398-9995.1997.tb02594.x.
Anisakis simplex larvae parasitize animals used as seafood and can produce a specific immune response in man. The ingestion of seafood contaminated with stage three of A. simplex larvae can induce a specific IgE response with clinical symptoms, usually urticaria, even if the fish is cooked before ingestion and the invasive infestation power destroyed by heating. Our preliminary studies showed a strong association of A. simplex sensitization with Ascaris lumbricoides, Daphnia, chironomid spp., Atlantic shrimp (Pandalus borealis), and German cockroach (Blattella germanica). We conducted the cross-reactivity study with cockroach, a ubiquitous insect, and Chironomidae (red mosquito larvae), a work-related allergen, without any possibility of Anisakis contamination. Serum samples were collected from 60 pediatric patients, with serum specific IgE to A. simplex. Both specific-IgE and immunoblot-inhibition studies, with a serum pool from 18 patients, were performed to determine whether the association of sensitizations to nematodes and arthropods was due to immunologic cross-reactivity. In addition, serum samples from 21 of 60 patients who showed also sensitization to German cockroach were used for individual immunoblot studies. In the serum pool, dose-dependent inhibition of B. germanica and Chironomus spp, was observed after preincubation with the A. simplex extract. Immunoblot of Anisakis, inhibited with Chironomus and German cockroach, yielded a partial blot inhibition but mainly on bands below 41 kDa. Blot inhibition of German cockroach and Chironomus with Anisakis was dose related. The band patterns in individual blots were heterogeneous, but most of them had bands of 30-43 kDa. None of these sera recognized allergens in the 14-kDa area. In our study, CAP-inhibition and immunoblot-inhibition analysis of Anisakis showed that several IgE-binding components could be shared by the three allergens.
简单异尖线虫幼虫寄生于用作海鲜的动物体内，并可在人体内产生特异性免疫反应。摄入被简单异尖线虫三期幼虫污染的海鲜可诱发伴有临床症状（通常为荨麻疹）的特异性IgE反应，即便该鱼在食用前已烹饪且加热已破坏其侵袭力。我们的初步研究表明，简单异尖线虫致敏与蛔虫、水蚤、摇蚊科物种、大西洋虾（北方长额虾）及德国小蠊之间存在密切关联。我们对一种常见昆虫蟑螂以及一种与工作相关的过敏原摇蚊科（红蚊子幼虫）进行了交叉反应性研究，不存在任何简单异尖线虫污染的可能性。从60名患有简单异尖线虫血清特异性IgE的儿科患者中采集血清样本。采用来自18名患者的血清池进行特异性IgE和免疫印迹抑制研究，以确定对线虫和节肢动物的致敏关联是否归因于免疫交叉反应。此外，从60名对德国小蠊也呈致敏反应的患者中的21名患者采集血清样本用于个体免疫印迹研究。在血清池中，用简单异尖线虫提取物预孵育后，观察到德国小蠊和摇蚊科物种的剂量依赖性抑制。用摇蚊和德国小蠊抑制的简单异尖线虫免疫印迹产生了部分印迹抑制，但主要是在41 kDa以下的条带。用简单异尖线虫对德国小蠊和摇蚊的印迹抑制呈剂量相关。个体印迹中的条带模式各异，但大多数都有30 - 43 kDa的条带。这些血清均未识别出14 kDa区域的过敏原。在我们的研究中，简单异尖线虫的CAP抑制和免疫印迹抑制分析表明，三种过敏原可能共享几种IgE结合成分。
