马动脉炎病毒的血凝反应。

Hemagglutination with equine arteritis virus.

作者信息

Kubota T, Inaba Y, Uwatoko K, Akashi H, Fukunaga Y

机构信息

Department of Veterinary Epizootiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.

出版信息

J Vet Med Sci. 1997 Oct;59(10):943-5. doi: 10.1292/jvms.59.943.

DOI:10.1292/jvms.59.943

PMID:9362047

Abstract

Equine arteritis virus (EAV) grown on RK13 cell cultures was tested for hemagglutination (HA) with erythrocytes from a variety of species at 4 degrees C, room temperature and 37 degrees C. HA was observed at all temperatures with erythrocytes from mouse and chicken but not with those of cattle, horse, rabbit, guinea pig, mongolian gerbil, goose or chick embryo. Chickens showed an individual variation in agglutinability of their erythrocytes, requiring selection of birds to obtain erythrocytes for HA. The HA activity was enhanced by treatment of virus materials with Tween 80 followed by treatment with ether. The HA reaction was inhibited by specific antiserum. Higher HA-inhibiting (HI) antibody titers were obtained by the incubation of serum-HA antigen mixture at 4 degrees C for 24 hr. HI antibody titers of individual horse sera showed a significant positive correlation with their neutralizing antibody titers.

摘要

在RK13细胞培养物上培养的马动脉炎病毒（EAV），在4℃、室温及37℃下，用来自多种物种的红细胞进行血凝试验（HA）。在所有温度下，用小鼠和鸡的红细胞均观察到血凝现象，而牛、马、兔、豚鼠、蒙古沙鼠、鹅或鸡胚的红细胞则未出现血凝现象。鸡的红细胞凝集性存在个体差异，需要挑选鸡以获取用于血凝试验的红细胞。用吐温80处理病毒材料后再用乙醚处理，可增强血凝活性。血凝反应被特异性抗血清抑制。血清-血凝抗原混合物在4℃孵育24小时可获得更高的血凝抑制（HI）抗体效价。个体马血清的HI抗体效价与其中和抗体效价呈显著正相关。