Makabe T, Sato M, Ouneda S, Inaba Y
Arch Virol. 1986;90(1-2):153-8. doi: 10.1007/BF01314153.
The virus was grown in MA 104 cells, a stable cell line derived from embryonic rhesus monkey kidney, and tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, room temperature and 37 degrees C. HA was observed at all temperatures with chicken, sheep, rabbit, guinea pig and human erythrocytes but not with horse, cattle, goat, swine and goose erythrocytes. HA reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were worked out. The HI test as well as neutralization test clearly distinguished ovine rotavirus from bovine, human, simian, equine, porcine and lapine rotaviruses.
该病毒在MA 104细胞中培养，MA 104细胞是一种源自恒河猴胚胎肾的稳定细胞系，并在4℃、室温及37℃下用多种物种的红细胞检测其血凝作用（HA）。在所有温度下，鸡、绵羊、兔、豚鼠和人红细胞均观察到HA，但马、牛、山羊、猪和鹅红细胞未观察到HA。HA反应被特异性抗血清抑制。对一些参与HA和血凝抑制（HI）的因素进行了研究，并制定了标准的HA和HI试验。HI试验以及中和试验能够清楚地区分绵羊轮状病毒与牛、人、猴、马、猪和兔轮状病毒。
