Tetsu N, Inaba Y, Yukawa M, Yoshiki K, Hirahara T, Furuya Y, Ito S, Yonemochi A, Ishikawa H
Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Arch Virol. 1989;106(3-4):321-6. doi: 10.1007/BF01313960.
Pseudorabies virus grown in CPK cell cultures was tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, 25 degrees C and 37 degrees C. HA was observed at all temperatures with mouse erythrocytes but not with cattle, sheep, goat, swine, cat, rabbit, guinea pig, rat, mongolian gerbil, chicken, and goose erythrocytes. Mice showed a strain variation in agglutinability of their erythrocytes, requiring selection of mice to obtain erythrocytes for HA. The HA reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were established. HI antibody titers of individual pig sera showed a significant positive correlation with their neutralizing antibody titers.
对在CPK细胞培养物中培养的伪狂犬病病毒，在4℃、25℃和37℃下用多种动物的红细胞进行血凝（HA）试验。在所有温度下，均观察到该病毒能凝集小鼠红细胞，但不能凝集牛、羊、山羊、猪、猫、兔、豚鼠、大鼠、蒙古沙鼠、鸡和鹅的红细胞。小鼠红细胞的凝集性存在毒株差异，需要挑选小鼠以获取用于HA试验的红细胞。血凝反应可被特异性抗血清抑制。对血凝及血凝抑制（HI）所涉及的一些因素进行了研究，并建立了标准的HA和HI试验。个体猪血清的HI抗体效价与其中和抗体效价呈显著正相关。
