Kurane I, Innis B L, Hoke C H, Eckels K H, Meager A, Janus J, Ennis F A
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
J Clin Lab Immunol. 1995;46(1):35-40.
It is accepted that T cells play a critical role during virus infections; however, T cell responses in vivo in acute stage of virus infection are not understood. We examined T cell activation in vivo in two volunteers who developed dengue fever in response to vaccination with a candidate live dengue vaccine. Serial plasma collected from the volunteers from day 0 (before infection) to day 17 after infection were examined for levels of soluble interleukin-2 receptor (sIL-2R), soluble CD4 (sCD4), soluble CD8 (sCD8), interleukin-2 (IL-2) and interferon gamma (INF gamma). Elevation of the levels of sIL-2R, IFN gamma, sCD4 and IL-2 became obvious during the period of viremia and was followed by a later increase in the level of sCD8. The levels of IFN gamma and sIL-2R declined after the end of the period of viremia. These results indicate that i. T cells are activated in vivo by dengue virus infection ii. activation of CD4+ T cells occurs during the period of viremia iii. activation of CD8+ T cells follows CD4+ T cell activation. These results suggest that activation of T cells in vivo may contribute to controlling acute dengue virus infections.
人们普遍认为T细胞在病毒感染过程中起着关键作用；然而，病毒感染急性期体内的T细胞反应尚不清楚。我们检测了两名接种候选登革热活疫苗后感染登革热的志愿者体内的T细胞活化情况。检测了从感染前第0天到感染后第17天从志愿者采集的系列血浆中可溶性白细胞介素-2受体（sIL-2R）、可溶性CD4（sCD4）、可溶性CD8（sCD8）、白细胞介素-2（IL-2）和干扰素γ（INFγ）的水平。在病毒血症期间，sIL-2R、IFNγ、sCD4和IL-2水平明显升高，随后sCD8水平稍后升高。病毒血症期结束后，IFNγ和sIL-2R水平下降。这些结果表明：i. 登革热病毒感染可在体内激活T细胞；ii. CD4+T细胞在病毒血症期被激活；iii. CD8+T细胞的激活发生在CD4+T细胞激活之后。这些结果提示，体内T细胞的激活可能有助于控制急性登革热病毒感染。
