Matsuoka M, Hamada K, Saika T, Mizobuti N, Takahashi I
Kochi Municipal Central Hospital, Department of Central Laboratory.
Rinsho Ketsueki. 1997 Sep;38(9):727-33.
Apoptosis of lymphocytes in infectious mononucleosis (IM) was studied. Apoptotic cells (APCs) were defined by morphological characteristics, such as nuclear fragmentation, undergoing apoptosis. Among 10 of 27 IM patients (37.0%) APCs > or = 3 per 500 peripheral white blood cells were observed on admission, but not in normal individuals. Sequential changes of proportions of both APCs and atypical lymphocytes (examined in 2 cases) showed a similar pattern, although APCs began to appear somewhat later and peak level of APCs were lower in comparison with those of atypical lymphocytes. APCs were positive for both anti-T cell and anti-Fas antigen monoclonal antibodies. Fas-positive cells were frequent in the early stage of IM, and returned to the normal range with the decrease of atypical lymphocytes. Sequential analyses of DNA fragmentation, done in one case, revealed that DNA fragmentation appeared with increase of APCs and disappeared with the decrease of APCs. These results suggest that apoptosis of T-cells in IM participates in the reconstitution of a proportion of lymphocytes disturbed by EB virus.
对传染性单核细胞增多症（IM）患者淋巴细胞凋亡进行了研究。凋亡细胞（APC）通过形态学特征来定义，如核碎裂，即正在经历凋亡。在27例IM患者中有10例（37.0%）在入院时观察到每500个外周血白细胞中凋亡细胞≥3个，而正常个体中未观察到。凋亡细胞和异型淋巴细胞比例的连续变化（对2例患者进行了检测）显示出相似的模式，尽管凋亡细胞开始出现的时间稍晚，且凋亡细胞的峰值水平与异型淋巴细胞相比更低。凋亡细胞对抗T细胞和抗Fas抗原单克隆抗体均呈阳性。Fas阳性细胞在IM早期较为常见，并随着异型淋巴细胞数量的减少而恢复到正常范围。对1例患者进行的DNA片段化连续分析显示，DNA片段化随着凋亡细胞数量的增加而出现，随着凋亡细胞数量的减少而消失。这些结果表明，IM中T细胞凋亡参与了受EB病毒干扰的淋巴细胞比例的重建。
