Crawford D H, Rickinson A B, Finerty S, Epstein M A
J Gen Virol. 1978 Mar;38(3):449-60. doi: 10.1099/0022-1317-38-3-449.
Experiments have been performed to identify the type and size of cell infected by EB virus in the blood of acute infectious mononucleosis (IM) patients, and to investigate the nature of the infection. Virus-infected cells, recognized by their ability to give rise to lymphoblastoid cell lines when co-cultivated with foetal lymphocytes, were shown to be restricted to the B-lymphocyte population. Samples of this population from each of eight IM patients were found to be negative for EB nuclear antigen (EBNA) staining. Thereafter, fractions of IM B-lymphocytes prepared on the basis of cell size were assayed either by co-cultivation, for the incidence of virus-infected cells, or by immunofluorescence staining for the presence of cells expressing EBNA. The great majority of virus-infected cells were found in the fractions of normal sized B-lymphocytes and yet these fractions were unequivocally EBNA-negative B-cell populations in IM blood is discussed in terms of the type of infection established by EB virus in the circulation of IM patients.
已开展实验以鉴定急性传染性单核细胞增多症（IM）患者血液中被EB病毒感染的细胞类型和大小，并研究感染的性质。当与胎儿淋巴细胞共同培养时，具有产生淋巴母细胞系能力的病毒感染细胞被证明仅限于B淋巴细胞群体。发现来自8名IM患者中每一位的该群体样本经EB核抗原（EBNA）染色均为阴性。此后，基于细胞大小制备的IM B淋巴细胞组分，通过共同培养检测病毒感染细胞的发生率，或通过免疫荧光染色检测表达EBNA的细胞的存在情况。发现绝大多数病毒感染细胞存在于正常大小B淋巴细胞的组分中，但这些组分在IM血液中明确是EBNA阴性B细胞群体，本文根据EB病毒在IM患者循环中建立的感染类型进行了讨论。
