Wang J, Cai X, Rivas G, Shiraishi H, Dontha N
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA.
Biosens Bioelectron. 1997;12(7):587-99. doi: 10.1016/s0956-5663(96)00076-0.
Wide-scale DNA testing requires the development of fast, small, easy-to-use biosensing devices. Various synthetic oligonucleotides and DNA have thus been immobilized onto microfabricated thick-film carbon transducers for performing several new nucleic-acid assay protocols. These include hybridization detection of nucleic acid sequences, determination of small molecules (drugs, pollutants) based on their collection into the dsDNA layer or via monitoring their effect upon the intrinsic DNA oxidation signal, and direct adsorptive stripping measurements of ultratrace levels of nucleic acids. Transduction of these DNA recognition processes is accomplished by a new highly-sensitive constant-current stripping chronopotentiometric operation. Comparison to traditional electrodes indicates that the biosensing performance is not compromised by the use of mass-producible disposable transducers. Such thick-film DNA biosensors have been coupled to a compact, user-friendly, hand-held analyzer. Applicability for the detection of sequences from M. tuberculosis and HIV-1 DNAs is illustrated. Such activity in the author's laboratory, aimed at developing DNA-coated screen-printed electrodes, is reviewed.
大规模DNA检测需要开发快速、小型、易于使用的生物传感设备。因此，各种合成寡核苷酸和DNA已被固定在微制造的厚膜碳传感器上，以执行几种新的核酸检测方案。这些方案包括核酸序列的杂交检测、基于小分子（药物、污染物）在双链DNA层中的聚集或通过监测它们对内在DNA氧化信号的影响来测定小分子，以及超痕量核酸的直接吸附溶出测量。这些DNA识别过程的转导是通过一种新型高灵敏度恒电流溶出计时电位操作来完成的。与传统电极的比较表明，使用可大量生产的一次性传感器不会损害生物传感性能。这种厚膜DNA生物传感器已与紧凑、用户友好的手持式分析仪相连。文中展示了其对结核分枝杆菌和HIV-1 DNA序列检测的适用性。本文综述了作者实验室在开发DNA涂层丝网印刷电极方面的此类活动。
