Marrazza G, Chianella I, Mascini M
Dipartimento di Sanità Pubblica, Epidemiologia, Chimica Analitica Ambientale, Sez. Chimica Analitica, Firenze, Italy.
Biosens Bioelectron. 1999 Jan 1;14(1):43-51. doi: 10.1016/s0956-5663(98)00102-x.
A disposable electrochemical sensor for the detection of short DNA sequences is described. Synthetic single-stranded oligonucleotides have been immobilized onto graphite screen printed electrodes with two procedures, the first involving the binding of avidinbiotinylated oligonucleotide and the second adsorption at a controlled potential. The probes were hybridized with different concentrations of complementary sequences. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry and chronopotentiometric stripping analysis using daunomycin hydrochloride as indicator of hybridization reaction. The probe immobilization step, the hybridization event and the indicator detection, have been optimized. The DNA sensor obtained by adsorption at a controlled potential was able to detect 1 microgram/ml of target sequence in the buffer solution using chronopotentiometric stripping analysis.
描述了一种用于检测短DNA序列的一次性电化学传感器。通过两种方法将合成的单链寡核苷酸固定在石墨丝网印刷电极上，第一种方法涉及抗生物素蛋白 - 生物素化寡核苷酸的结合，第二种方法是在可控电位下进行吸附。将探针与不同浓度的互补序列杂交。使用盐酸柔红霉素作为杂交反应的指示剂，通过差分脉冲伏安法和计时电位溶出分析法评估电极表面形成的杂交体。对探针固定步骤、杂交事件和指示剂检测进行了优化。通过在可控电位下吸附获得的DNA传感器，使用计时电位溶出分析法能够在缓冲溶液中检测到1微克/毫升的靶序列。
