Grimm R, Zanaboni G, Viglio S, Dyne K M, Cetta G, Iadarola P
Analytical Division, Hewlett-Packard, Waldbronn, Germany.
J Chromatogr B Biomed Sci Appl. 1997 Sep 26;698(1-2):47-57. doi: 10.1016/s0378-4347(97)00309-5.
Prolidase deficiency is a severe disorder characterized by massive excretion of metabolites with closely related structures. At present, micellar electrokinetic chromatography is the separation method which provides the highest selectivity of structurally similar solutes. However, the structure of a surfactant can greatly affect the selectivity of separation depending on factors such as the length of hydrophobic alkyl chain or the nature of the hydrophilic group. Here we investigated the effect of three non-ionic and four anionic detergents for obtaining the best separation conditions for resolving imidodipeptide mixtures. The effect on resolution of variables such as temperature, surfactant concentrations and organic solvents was also examined. The greatest resolution was obtained at the lowest temperature studied (10 degrees C) using 50 mM sodium borate, pH 9.3 containing 50 mM pentanesulfonate and 10% (v/v) methanol. Under these experimental conditions almost all excreted components were baseline separated and identified.
脯氨酰二肽氨基肽酶缺乏症是一种严重的疾病，其特征是大量排泄结构密切相关的代谢物。目前，胶束电动色谱法是对结构相似溶质具有最高选择性的分离方法。然而，表面活性剂的结构会极大地影响分离的选择性，这取决于诸如疏水烷基链长度或亲水基团性质等因素。在此，我们研究了三种非离子型和四种阴离子型去污剂对获得最佳分离条件以解析亚氨基二肽混合物的影响。还考察了温度、表面活性剂浓度和有机溶剂等变量对分离度的影响。在研究的最低温度（10℃）下，使用含有50 mM戊烷磺酸盐和10%（v/v）甲醇的50 mM硼酸钠（pH 9.3），获得了最大分离度。在这些实验条件下，几乎所有排泄成分都实现了基线分离并得以鉴定。
