Benmansour A, Basurco B, Monnier A F, Vende P, Winton J R, de Kinkelin P
Institut National de la Recherche Agronomique, Unité de Virologie et Immunologie Moléculaires, Jouy-en-Josas, France.
J Gen Virol. 1997 Nov;78 ( Pt 11):2837-46. doi: 10.1099/0022-1317-78-11-2837.
To evaluate the genetic diversity of viral haemorrhagic septicaemia virus (VHSV), the sequence of the glycoprotein genes (G) of 11 North American and European isolates were determined. Comparison with the G protein of representative members of the family Rhabdoviridae suggested that VHSV was a different virus species from infectious haemorrhagic necrosis virus (IHNV) and Hirame rhabdovirus (HIRRV). At a higher taxonomic level, VHSV, IHNV and HIRRV formed a group which was genetically closest to the genus Lyssavirus. Compared with each other, the G genes of VHSV displayed a dissimilar overall genetic diversity which correlated with differences in geographical origin. The multiple sequence alignment of the complete G protein, showed that the divergent positions were not uniformly distributed along the sequence. A central region (amino acid position 245-300) accumulated substitutions and appeared to be highly variable. The genetic heterogeneity within a single isolate was high, with an apparent internal mutation frequency of 1.2 x 10(-3) per nucleotide site, attesting the quasispecies nature of the viral population. The phylogeny separated VHSV strains according to the major geographical area of isolation: genotype I for continental Europe, genotype II for the British Isles, and genotype III for North America. Isolates from continental Europe exhibited the highest genetic variability, with sub-groups correlated partially with the serological classification. Neither neutralizing polyclonal sera, nor monoclonal antibodies, were able to discriminate between the genotypes. The overall structure of the phylogenetic tree suggests that VHSV genetic diversity and evolution fit within the model of random change and positive selection operating on quasispecies.
为评估病毒性出血性败血症病毒（VHSV）的遗传多样性，测定了11株北美和欧洲分离株的糖蛋白基因（G）序列。与弹状病毒科代表性成员的G蛋白比较表明，VHSV是一种不同于传染性出血性坏死病毒（IHNV）和牙鲆弹状病毒（HIRRV）的病毒种。在更高的分类水平上，VHSV、IHNV和HIRRV形成了一个在遗传上最接近狂犬病病毒属的类群。相互比较时，VHSV的G基因显示出不同的总体遗传多样性，这与地理起源的差异相关。完整G蛋白的多序列比对表明，分歧位点在序列上并非均匀分布。一个中央区域（氨基酸位置245 - 300）积累了替换，似乎高度可变。单个分离株内的遗传异质性很高，每个核苷酸位点的表观内部突变频率为1.2×10⁻³，证明了病毒群体的准种性质。系统发育根据主要分离地理区域将VHSV毒株分开：欧洲大陆为基因型I，不列颠群岛为基因型II，北美为基因型III。来自欧洲大陆的分离株表现出最高的遗传变异性，亚群部分与血清学分类相关。中和多克隆血清和单克隆抗体均无法区分这些基因型。系统发育树的总体结构表明，VHSV的遗传多样性和进化符合对准种进行随机变化和正选择的模型。
