用4',6-二脒基-2-苯基吲哚（DAPI）对出现在各种细胞类型上及细胞内的酸性粘多糖进行染色。
Staining of acid mucopolysaccharides appearing on and in various cell types by DAPI.
作者信息
Grossgebauer K
出版信息
Microsc Acta. 1979 Nov;82(3):291-3.
PMID:93685
Abstract
It could be shown that DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole), is able to stain various types of acid mucopolysaccharides, e.g. heparin and agar mucopolysaccharides. Due to their content of heparin, mast cells and basophil cells could be stained rapidly. Other cells e.g. mononuclear phagocytes and malignant epithelial cells, could be loaded with these acid mucopolysaccharides and subsequently stained by DAPI.
摘要
可以证明，新型荧光染料4',6-二脒基-2-苯基吲哚（DAPI）能够对多种酸性粘多糖进行染色，例如肝素和琼脂粘多糖。由于肥大细胞和嗜碱性粒细胞含有肝素，因此可以快速被染色。其他细胞，如单核吞噬细胞和恶性上皮细胞，能够摄取这些酸性粘多糖，随后被DAPI染色。
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