Clemens M R, Waladkhani A R, Bublitz K, Ehninger G, Gey K F
Krankenanstalt Mutterhaus der Borromäerinnen, Trier, Federal Republic of Germany.
Wien Klin Wochenschr. 1997 Oct 17;109(19):771-6.
Conditioning therapy preceding bone marrow transplantation (BMT) usually consists of high-dose chemotherapy and total body irradiation (TBI). It has acute and delayed toxic effects on several tissues, possibly related to peroxidation processes and exhaustion of antioxidants. Early studies indicated an increase of peroxide processes and a decrease of antioxidants during conditioning therapy. Hence, we investigated the effect of antioxidant supplementation on peroxidation processes and antioxidant status. We supplemented a patient group (N = 16) [BMT (+)], with oral 45 mg beta-carotene, 825 mg alpha-tocopherol and 450 mg ascorbic acid daily for three weeks before conditioning therapy. Another patient group (N = 10), BMT(-), was not supplemented with antioxidants before conditioning therapy. In order to investigate the physiologic effect of supplement antioxidants a healthy control group (N = 10) was supplemented with the same doses as BMT(+). Peroxide concentrations in plasma were measured by using the cholesterol oxidase (CHOD)-iodide method and antioxidants were measured by HPLC. Before supplementation the beta-carotene and alpha-tocopherol concentrations were comparable in both patient groups. After supplementation significantly higher beta-carotene and alpha-tocopherol concentrations were measured in the supplemented patients, BMT(+), than in the unsupplemented patients, BMT(-). After conditioning therapy, BMT(+) patients showed a significantly higher beta-carotene concentration (p < 0.05) than before supplementation. In BMT(-) patients the beta-carotene (p < 0.05) and alpha-tocopherol concentrations (p < 0.01) decreased significantly and the lipid peroxide concentration increased significantly following conditioning therapy. We conclude that antioxidant supplementation prior to conditioning therapy reduces peroxidation processes induced by conditioning therapy in bone marrow recipients.
骨髓移植（BMT）前的预处理疗法通常包括大剂量化疗和全身照射（TBI）。它对多个组织具有急性和延迟毒性作用，这可能与过氧化过程和抗氧化剂耗竭有关。早期研究表明，预处理疗法期间过氧化物生成增加而抗氧化剂减少。因此，我们研究了补充抗氧化剂对过氧化过程和抗氧化状态的影响。我们对一组患者（N = 16）[BMT（+）组]在预处理疗法前三周每天口服45毫克β-胡萝卜素、825毫克α-生育酚和450毫克抗坏血酸。另一组患者（N = 10），即BMT（-）组，在预处理疗法前未补充抗氧化剂。为了研究补充抗氧化剂的生理效应，我们对一个健康对照组（N = 10）给予与BMT（+）组相同剂量的抗氧化剂。采用胆固醇氧化酶（CHOD）-碘化物法测定血浆中的过氧化物浓度，并用高效液相色谱法测定抗氧化剂含量。在补充之前，两组患者的β-胡萝卜素和α-生育酚浓度相当。补充后，补充了抗氧化剂的BMT（+）组患者的β-胡萝卜素和α-生育酚浓度显著高于未补充的BMT（-）组患者。预处理疗法后，BMT（+）组患者的β-胡萝卜素浓度显著高于补充前（p < 0.05）。在BMT（-）组患者中，预处理疗法后β-胡萝卜素（p < 0.05）和α-生育酚浓度（p < 0.01）显著降低，脂质过氧化物浓度显著升高。我们得出结论，预处理疗法前补充抗氧化剂可减少骨髓移植受者因预处理疗法诱导的过氧化过程。
