Erhardt Jürgen G, Mack Heidrun, Sobeck Ulrike, Biesalski Hans K
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany.
Br J Nutr. 2002 May;87(5):471-5. doi: 10.1079/BJNBJN2002566.
The uptake of alpha-tocopherol and beta-carotene and their antioxidative effect in plasma and buccal mucosal cells after oral application in twelve subjects is demonstrated in our study. The effect on the antioxidative status was evaluated using a modified thiobarbituric acid-reactive substance (TBARS) method. As expected, the supplement of 134.2 mg alpha-tocopherol/d and 25 mg beta-carotene/d for 7 d resulted in a significant increase of alpha-tocopherol and beta-carotene concentration in plasma (P<0.05). In buccal mucosal cells, the concentration of beta-carotene increased after supplementation (P<0.05), whereas the concentration of alpha-tocopherol remained constant. A decrease in TBARS (P<0.05) was found in buccal mucosal cells but not in plasma. In conclusion, an uptake of the supplemented antioxidants was detected in plasma and in buccal mucosal cells. There was significant change in beta-carotene concentration and oxidative stress as measured using a modified TBARS test in buccal mucosal cells, but not in the plasma.
我们的研究表明了12名受试者口服α-生育酚和β-胡萝卜素后，它们在血浆和颊黏膜细胞中的摄取情况及其抗氧化作用。使用改良的硫代巴比妥酸反应性物质（TBARS）方法评估对抗氧化状态的影响。正如预期的那样，连续7天每天补充134.2毫克α-生育酚和25毫克β-胡萝卜素导致血浆中α-生育酚和β-胡萝卜素浓度显著增加（P<0.05）。在颊黏膜细胞中，补充后β-胡萝卜素浓度增加（P<0.05），而α-生育酚浓度保持不变。在颊黏膜细胞中发现TBARS降低（P<0.05），但在血浆中未发现。总之，在血浆和颊黏膜细胞中检测到补充抗氧化剂的摄取。使用改良的TBARS试验测量，颊黏膜细胞中的β-胡萝卜素浓度和氧化应激有显著变化，但血浆中没有。
