Sugiyama T, Kido N, Kato Y, Koide N, Yoshida T, Yokochi T
Department of Microbiology and Immunology, Aichi Medical University, Nagakute, Japan.
Gene. 1997 Oct 1;198(1-2):111-3. doi: 10.1016/s0378-1119(97)00300-4.
In order to clarify the evolutionary relationship among rfb gene clusters synthesizing mannose homopolymer as O-specific polysaccharides in Escherichia coli and Klebsiella, we studied the DNA sequence of the boundary region between the rfb and his genes in a series of strains possessing mannose homopolymer as O-specific polysaccharide. All had a characteristic gene organization carrying no gene between the rfb and his genes. Further, the recombination event was suggested to occur at the same site of the hisI gene in those strains. It was suggested that there was a close evolutionary relationship among rfb gene clusters synthesizing mannose homopolymer as O-specific polysaccharide in E. coli and Klebsiella.
为了阐明在大肠杆菌和克雷伯氏菌中合成作为O-特异性多糖的甘露糖同聚物的rfb基因簇之间的进化关系，我们研究了一系列具有作为O-特异性多糖的甘露糖同聚物的菌株中rfb和his基因之间边界区域的DNA序列。所有菌株都具有特征性的基因组织，在rfb和his基因之间没有基因。此外，提示在这些菌株中重组事件发生在hisI基因的同一位置。这表明在大肠杆菌和克雷伯氏菌中合成作为O-特异性多糖的甘露糖同聚物的rfb基因簇之间存在密切的进化关系。
