以甘露糖均聚物作为O-特异性多糖的重组脂多糖增强的免疫活性。
Augmented immunological activities of recombinant lipopolysaccharide possessing the mannose homopolymer as the O-specific polysaccharide.
作者信息
Paeng N, Kido N, Schmidt G, Sugiyama T, Kato Y, Koide N, Yokochi T
机构信息
Department of Natural Science Informatics, School of Informatics and Sciences, Nagoya University, Aichi, Japan.
出版信息
Infect Immun. 1996 Jan;64(1):305-9. doi: 10.1128/iai.64.1.305-309.1996.
Recombinant lipopolysaccharide possessing the mannose homopolymer as the O-specific polysaccharide was manufactured genetically by transforming Escherichia coli K-12 with various rfb genes capable of synthesizing the mannose homopolymer. Recombinant lipopolysaccharide exhibited levels of anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity much higher than those exhibited by the original rough-type lipopolysaccharide from E. coli K-12 or lipopolysaccharide possessing the heteropolysaccharide from E. coli O111. Immunological activities of recombinant lipopolysaccharide were as strong as those of wild-type lipopolysaccharide possessing the mannose homopolymer. Characteristic activities of wild-type lipolysaccharide possessing the mannose homopolymer were exhibited by recombinant lipopolysaccharide. The abilities of lipopolysaccharide to activate B cells polyclonally and to produce cytokines did not seem to be related to the presence of the mannose homopolymer. Therefore, it was suggested that the mannose homopolymer in the O-specific polysaccharide might exclusively enhance anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity among various lipid A activities.
通过用各种能够合成甘露糖同聚物的rfb基因转化大肠杆菌K - 12，从基因层面制造出了以甘露糖同聚物作为O - 特异性多糖的重组脂多糖。重组脂多糖表现出的抗补体活性、佐剂活性和局部淋巴结肿大活性水平，比大肠杆菌K - 12的原始粗糙型脂多糖或含有大肠杆菌O111杂多糖的脂多糖所表现出的水平要高得多。重组脂多糖的免疫活性与含有甘露糖同聚物的野生型脂多糖的免疫活性一样强。重组脂多糖表现出了含有甘露糖同聚物的野生型脂多糖的特征活性。脂多糖多克隆激活B细胞和产生细胞因子的能力似乎与甘露糖同聚物的存在无关。因此，有人提出，在各种脂多糖A活性中，O - 特异性多糖中的甘露糖同聚物可能专门增强抗补体活性、佐剂活性和局部淋巴结肿大活性。
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