一个与剪接因子spU2AF59相互作用的新型裂殖酵母基因spUAP2的分子特征分析
Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.
作者信息
McKinney R, Wentz-Hunter K, Schmidt H, Potashkin J
机构信息
Department of Pharmacology and Molecular Biology, Finch University of Health Sciences, The Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA.
出版信息
Curr Genet. 1997 Nov;32(5):323-30. doi: 10.1007/s002940050284.
A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.
一种前体信使核糖核酸（pre-mRNA）剪接所必需的蛋白质，即U2辅助因子（U2AF），由一个大亚基和一个小亚基组成。此前我们从裂殖酵母中克隆并鉴定了这两个亚基，即裂殖酵母U2AF59和裂殖酵母U2AF23。我们现在报告一种新的U2AF相关蛋白，即裂殖酵母UAP2，它与这两个亚基都相互作用。裂殖酵母UAP2包含一个经典的和一个退化的RNA识别基序（RRM），这两者都是与裂殖酵母U2AF59相互作用所必需的。相互作用还需要裂殖酵母U2AF59的富含精氨酸/丝氨酸的区域和第一个RRM。裂殖酵母UAP2基因的无效等位基因是致死的。
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