裂殖酵母中的剪接体前体形成需要SF1-U2AF(59)-U2AF(23)的稳定复合物。
Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).
作者信息
Huang Tao, Vilardell Josep, Query Charles C
机构信息
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461-1975, USA.
出版信息
EMBO J. 2002 Oct 15;21(20):5516-26. doi: 10.1093/emboj/cdf555.
Abstract
We have initiated a biochemical analysis of splicing complexes in extracts from the fission yeast Schizosaccharomyces pombe. Extracts of S.pombe contain high levels of the spliceosome-like U2/5/6 tri-snRNP, which dissociates into mono-snRNPs in the presence of ATP, and supports binding of U2 snRNP to the 3' end of introns, yielding a weak ATP-independent E complex and the stable ATP-dependent complex A. The requirements for S.pombe complex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U2AF) are analogous to those of mammalian complex A. The S.pombe SF1/BBP, U2AF(59) and U2AF(23) are tightly associated in a novel complex that is required for complex A formation. This pre-formed SF1- U2AF(59)-U2AF(23) complex may represent a streamlined mechanism for recognition of the branch site, pyrimidine tract and 3' splice site at the 3' end of introns.
摘要
我们已经开始对裂殖酵母粟酒裂殖酵母提取物中的剪接复合体进行生化分析。粟酒裂殖酵母提取物含有高水平的剪接体样U2/5/6三小核核糖核蛋白，其在ATP存在下会解离成单小核核糖核蛋白，并支持U2小核核糖核蛋白与内含子3'末端结合，产生一个弱的不依赖ATP的E复合体和稳定的依赖ATP的复合体A。粟酒裂殖酵母复合体A形成的要求（前体mRNA序列元件、蛋白质剪接因子、SF1/BBP和U2AF的两个亚基）与哺乳动物复合体A的要求相似。粟酒裂殖酵母的SF1/BBP、U2AF(59)和U2AF(23)紧密结合在一个新的复合体中，该复合体是复合体A形成所必需的。这种预先形成的SF1-U2AF(59)-U2AF(23)复合体可能代表了一种简化的机制，用于识别内含子3'末端的分支位点、嘧啶序列和3'剪接位点。
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本文引用的文献
1
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RNA. 2002 Apr;8(4):426-39. doi: 10.1017/s1355838202021088.
2
Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome.Prp43是一种必需的RNA依赖性ATP酶，是从剪接体中释放套索状内含子所必需的。
J Biol Chem. 2002 May 17;277(20):17743-50. doi: 10.1074/jbc.M200762200. Epub 2002 Mar 8.
3
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.蛋白质组学分析揭示了裂殖酵母和芽殖酵母中均存在稳定的多蛋白复合物，这些复合物包含与Myb相关的Cdc5p/Cef1p、新型前体mRNA剪接因子和小核RNA。
Mol Cell Biol. 2002 Apr;22(7):2011-24. doi: 10.1128/MCB.22.7.2011-2024.2002.
4
The genome sequence of Schizosaccharomyces pombe.粟酒裂殖酵母的基因组序列。
Nature. 2002 Feb 21;415(6874):871-80. doi: 10.1038/nature724.
5
Composition and functional characterization of the yeast spliceosomal penta-snRNP.酵母剪接体五聚体小核核糖核蛋白的组成与功能特性
Mol Cell. 2002 Jan;9(1):31-44. doi: 10.1016/s1097-2765(02)00436-7.
6
A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.一种与前体mRNA分支位点相关的新型U2和U11/U12核小核糖核蛋白（snRNP）蛋白。
EMBO J. 2001 Aug 15;20(16):4536-46. doi: 10.1093/emboj/20.16.4536.
7
The tandem affinity purification (TAP) method: a general procedure of protein complex purification.串联亲和纯化（TAP）方法：蛋白质复合物纯化的通用程序。
Methods. 2001 Jul;24(3):218-29. doi: 10.1006/meth.2001.1183.
8
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9
Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals.裂殖酵母中剪接机制的分析：与芽殖酵母和哺乳动物的比较。
Nucleic Acids Res. 2000 Aug 15;28(16):3003-10. doi: 10.1093/nar/28.16.3003.
10
Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure.U2AF大亚基中的突变会破坏前体mRNA剪接、细胞周期进程和核结构。
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