Mittag M, Waltenberger H
Botanisches Institut, Ludwig-Maximilians-Universität München, Germany.
Biol Chem. 1997 Oct;378(10):1167-70.
The luciferin-binding protein (LBP) from the dinoflagellate, Gonyaulax polyedra, is regulated by a circadian clock at the translational level. A 22-nucleotide long interval in the lbp 3' untranslated region, which contains seven UG-repeats, was characterized as a circadian cis-acting element, to which a clock controlled factor (CCTR) binds. Recently we have found that the phylogenetically distant green alga, Chlamydomonas reinhardtii, contains a CCTR analog, called Chlamy 1. Here we show that the flanking nucleotides surrounding the UG-repeats are required for high binding activity of CCTR and Chlamy 1. The absence of three or more UG-repeats abolishes binding with both proteins.
来自多甲藻（Gonyaulax polyedra）的荧光素结合蛋白（LBP）在翻译水平上受生物钟调控。lbp 3'非翻译区有一个22个核苷酸长的间隔区，其中包含七个UG重复序列，该区域被鉴定为昼夜节律顺式作用元件，生物钟控制因子（CCTR）可与之结合。最近我们发现，在系统发育上距离较远的绿藻莱茵衣藻（Chlamydomonas reinhardtii）中含有一种CCTR类似物，称为Chlamy 1。在此我们表明，UG重复序列周围的侧翼核苷酸是CCTR和Chlamy 1具有高结合活性所必需的。缺少三个或更多的UG重复序列会消除与这两种蛋白的结合。
