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昼夜节律调节的腰鞭毛虫荧光素酶的mRNA水平在整个周期中保持恒定。

The mRNA level of the circadian regulated Gonyaulax luciferase remains constant over the cycle.

作者信息

Mittag M, Li L, Hastings J W

机构信息

Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138-2020, USA.

出版信息

Chronobiol Int. 1998 Jan;15(1):93-8. doi: 10.3109/07420529808998673.

Abstract

The expression of luciferin-binding protein (LBP) and luciferase (LCF), two proteins that are involved in bioluminescence in Gonyaulax polyedra, is controlled by a cellular circadian clock. In the case of LBP, its temporal expression is reported to be regulated at the translational level, involving both 5' and 3' untranslated regions (UTRs) of lbp mRNA. Here, we show that the amounts of lcf mRNA are constant throughout the day-night cycle, indicating that the circadian expression of LCF is also regulated at the translational level.

摘要

多甲藻(Gonyaulax polyedra)中参与生物发光的两种蛋白质,即荧光素结合蛋白(LBP)和荧光素酶(LCF)的表达受细胞生物钟控制。就LBP而言,据报道其时间表达在翻译水平受到调控,涉及lbp mRNA的5'和3'非翻译区(UTR)。在这里,我们表明lcf mRNA的量在昼夜循环中保持恒定,这表明LCF的昼夜表达也在翻译水平受到调控。

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