Lamb V A, Dalton H P, Wilkins J R
Am J Clin Pathol. 1976 Jul;66(1):91-5. doi: 10.1093/ajcp/66.1.91.
A method for detecting the presence of bacteria in urine based on measuring a change in potential between two electrodes was tested in a clinical microbiology laboratory. Initial tests were conducted with 13 bacteria commonly associated with urinary-tract infections; all of the test organisms were detected within 2--9 hours. A linear relationship was established between inoculum size and the time an increase in voltage was observed on a strip-chart recorder. No response was seen with sterile urine, but urine samples inoculated with Escherichia produced the expected positive response. One hundred twenty-eight urine specimens from hospitalized persons were simultaneously tested by the electrochemical detection method (ECDM) and by conventional bacteriologic procedures. Ninety-four per cent of 49 positive samples with counts of 10(5) organisms per ml. or more were detected within 4 hours and 100% at 10 hours with the ECDM. Twenty-nine specimens with counts less than 10(5) cells per ml. were detected in 3.5 to 9 hours; two samples (8%) in this group were positive within 4 hours. Fifty samples were negative for bacterial growth, and no increase in voltage was found with these samples.
一种基于测量两个电极之间电位变化来检测尿液中细菌存在的方法，在临床微生物学实验室进行了测试。最初的测试使用了13种通常与尿路感染相关的细菌；所有测试菌株在2至9小时内都被检测到。在接种量与在条形图记录仪上观察到电压升高的时间之间建立了线性关系。无菌尿液无反应，但接种大肠杆菌的尿液样本产生了预期的阳性反应。对128份来自住院患者的尿液标本同时采用电化学检测法（ECDM）和传统细菌学方法进行检测。每毫升含10⁵个或更多细菌的49份阳性样本中，94%在4小时内被ECDM检测到，10小时时100%被检测到。每毫升含细菌数少于10⁵个的29份标本在3.5至9小时内被检测到；该组中有两份样本（8%）在4小时内呈阳性。50份样本细菌生长呈阴性，这些样本未发现电压升高。
