Dahms S E, Piechota H J, Nunes L, Dahiya R, Lue T F, Tanagho E A
Department of Urology, University of California School of Medicine, San Francisco, USA.
Urology. 1997 Nov;50(5):818-25. doi: 10.1016/S0090-4295(97)00391-9.
To evaluate ureteral replacement by a free homologous graft of acellular matrix in a rat model.
In 30 male Sprague-Dawley rats, a 0.3 to 0.8-cm midsegment of the left ureter was resected and replaced with an acellular matrix graft of equal length placed on a polyethylene stent. The animals were killed at varying intervals, and the grafted specimens were prepared for light and electron microscopy.
In all animals, the acellular matrix graft remained in its original position without evidence of incrustation or infection, and histologic examination showed complete epithelialization and progressive infiltration by vessels. At 10 weeks, smooth muscle fibers were observed; at 12 weeks, nerve fibers were first detected; at 4 months, smooth muscle cells had assumed regular configuration.
The ureteral acellular matrix graft appears to promote the regeneration of all ureteral wall components.
在大鼠模型中评估脱细胞基质的游离同种异体移植物替代输尿管的效果。
选取30只雄性Sprague-Dawley大鼠，切除左侧输尿管0.3至0.8厘米的中段，并用置于聚乙烯支架上的等长脱细胞基质移植物进行替代。在不同时间间隔处死动物，将移植标本制备用于光镜和电镜检查。
在所有动物中，脱细胞基质移植物均保留在原位，无结痂或感染迹象，组织学检查显示完全上皮化且血管逐渐浸润。10周时观察到平滑肌纤维；12周时首次检测到神经纤维；4个月时，平滑肌细胞呈现规则形态。
输尿管脱细胞基质移植物似乎能促进输尿管壁所有成分的再生。
