Takami Y, Matsuda T, Yoshitake M, Hanumadass M, Walter R J
Department of Plastic Surgery, Sapporo Medical College, Japan.
Burns. 1996 May;22(3):182-90. doi: 10.1016/0305-4179(95)00123-9.
A method for preparing acellular allogeneic dermal matrix (ADM) and its effectiveness as a dermal substitute are described. Treatment of rat skin with Dispase followed by Triton X-100 completely removed cellular components from the dermis. Subcutaneously implanted ADM evoked no immunological reaction and 20 weeks after implantation, the size of the implanted ADM was reduced to about 60 per cent of its original area. ADM became completely vascularized within 2 weeks after implantation into full thickness skin defects in the rat and inhibited extensive wound contracture. A second layer of ADM placed onto the implanted ADM served as an excellent dressing, providing mechanical protection and permitting vascularization of the underlying implant. Onlay skin autografts placed onto vascularized allogeneic ADM showed good survival when the skin was grafted more than 1 week after ADM implantation. Dispase/detergent treated ADM derived from animal or human skin may be useful in full thickness skin defects providing a vascularized bed for subsequent epidermal coverage.
本文描述了一种制备脱细胞异体真皮基质（ADM）的方法及其作为真皮替代物的有效性。用Dispase处理大鼠皮肤，随后用Triton X - 100处理，可完全去除真皮中的细胞成分。皮下植入的ADM未引发免疫反应，植入20周后，植入的ADM大小缩小至其原始面积的约60%。将ADM植入大鼠全层皮肤缺损后2周内完全血管化，并抑制了广泛的伤口挛缩。置于植入的ADM上的第二层ADM可作为优良的敷料，提供机械保护并使下层植入物血管化。当在ADM植入1周以上后移植皮肤时，置于血管化异体ADM上的自体皮肤移植显示出良好的存活情况。源自动物或人类皮肤经Dispase/去污剂处理的ADM，可用于全层皮肤缺损，为后续的表皮覆盖提供血管化床。
