将假定的转录修复偶联因子CSB/ERCC6招募至RNA聚合酶II延伸复合物。
Recruitment of the putative transcription-repair coupling factor CSB/ERCC6 to RNA polymerase II elongation complexes.
作者信息
Tantin D, Kansal A, Carey M
机构信息
Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095-1737, USA.
出版信息
Mol Cell Biol. 1997 Dec;17(12):6803-14. doi: 10.1128/MCB.17.12.6803.
Abstract
Cockayne's syndrome (CS) is a disease characterized by developmental and growth defects, sunlight sensitivity, and a defect in transcription-coupled nucleotide excision repair. The two principle proteins involved in CS, CSA and CSB/ERCC6, have been hypothesized to bind RNA polymerase II (Pol II) and link transcription to DNA repair. We have tested CSA and CSB in assays designed to determine their role in transcription-coupled repair. Using a unique oligo(dC)-tailed DNA template, we provide biochemical evidence that CSB/ERCC6 interacts with Pol II molecules engaged in ternary complexes containing DNA and nascent RNA. CSB is a DNA-activated ATPase, and hydrolysis of the ATP beta-gamma phosphoanhydride bond is required for the formation of a stable Pol II-CSB-DNA-RNA complex. Unlike CSB, CSA does not directly bind Pol II.
摘要
科凯恩综合征（CS）是一种以发育和生长缺陷、对阳光敏感以及转录偶联核苷酸切除修复缺陷为特征的疾病。参与CS的两种主要蛋白质CSA和CSB/ERCC6，据推测可结合RNA聚合酶II（Pol II）并将转录与DNA修复联系起来。我们在旨在确定它们在转录偶联修复中作用的实验中对CSA和CSB进行了测试。使用独特的寡聚（dC）尾DNA模板，我们提供了生化证据，证明CSB/ERCC6与参与包含DNA和新生RNA的三元复合物的Pol II分子相互作用。CSB是一种DNA激活的ATP酶，ATPβ-γ磷酸酐键的水解是形成稳定的Pol II-CSB-DNA-RNA复合物所必需的。与CSB不同，CSA不直接结合Pol II。
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