Akita H, Matsuyama T, Iso H, Sugita M, Yoshida S
Fifth Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
Brain Res. 1997 Sep 19;769(1):86-96. doi: 10.1016/s0006-8993(97)00674-4.
We evaluated the effects of oxidative stress in mouse brain induced by the intraperitoneal injection of diethyldithiocarbamate (DDC) on gene expression of the novel serine protease, neuropsin, and on shock-avoidance learning. The level of neuropsin mRNA in the hippocampal pyramidal neurons increased at 2 h after DDC treatment and decreased thereafter. At 7 days neuropsin mRNA significantly decreased to 60% of the pretreated control level and then returned to the control level at 30 days. Genes for tissue plasminogen activator, manganese superoxide dismutase, and heat shock protein did not differ in DDC-treated mice vs. the control group at 7 and 30 days. The shuttle-box avoidance learning was retarded at 7 days after DDC administration. However, it recovered to the control level at 30 days after DDC administration. The results suggest that generation of reactive oxygen species has an important role in neuropsin transcript in the limbic areas which might be related to the disturbance in avoidance learning.
我们评估了腹腔注射二乙二硫代氨基甲酸盐（DDC）诱导的小鼠脑氧化应激对新型丝氨酸蛋白酶神经蛋白酶基因表达以及对回避学习的影响。DDC处理后2小时，海马锥体神经元中的神经蛋白酶mRNA水平升高，此后下降。在7天时，神经蛋白酶mRNA显著降至预处理对照水平的60%，然后在30天时恢复到对照水平。在7天和30天时，DDC处理的小鼠与对照组相比，组织型纤溶酶原激活物、锰超氧化物歧化酶和热休克蛋白的基因没有差异。DDC给药后7天，穿梭箱回避学习受到阻碍。然而，在DDC给药后30天恢复到对照水平。结果表明，活性氧的产生在边缘区域的神经蛋白酶转录中起重要作用，这可能与回避学习障碍有关。
