Bertelli E, Bendayan M
Department of Anatomy, Université de Montréal, Quebec, Canada.
Am J Physiol. 1997 Nov;273(5):C1641-9. doi: 10.1152/ajpcell.1997.273.5.C1641.
When tissues were subjected to 24 h of duct ligation, intermediate pancreatic cells simultaneously displaying endocrine and exocrine phenotypes appeared. Immunocytochemistry by laser scanning confocal microscopy revealed the appearance of a large number of these cells coexpressing insulin and amylase. These cells were located within the islets of Langerhans as well as in the acinar parenchyma. They were also detected in a culture system of isolated pancreatic cells. With the use of immunoelectron microscopy, two types of secretory granules were identified in these cells. One was insulin immunoreactive, whereas the other, resembling zymogen granules, contained amylase. Occasionally, some small granules displayed a double labeling for both secretory proteins. Numerous crinophagic bodies and autophagosomes containing insulin and/or amylase were also present. In situ hybridization, applied with the specific probes, confirmed the presence of both insulin and amylase mRNAs in these cells. Because duct ligation is known to induce insulin cell proliferation, the present results confirm that endocrine-acinar cells do appear in such condition and may represent intermediate steps in a transdifferentiating process.
当组织进行24小时胆管结扎时，出现了同时显示内分泌和外分泌表型的胰腺中间细胞。通过激光扫描共聚焦显微镜进行的免疫细胞化学显示大量共表达胰岛素和淀粉酶的此类细胞出现。这些细胞位于胰岛以及腺泡实质内。在分离的胰腺细胞培养系统中也检测到了它们。利用免疫电子显微镜，在这些细胞中鉴定出两种类型的分泌颗粒。一种是胰岛素免疫反应性的，而另一种类似于酶原颗粒，含有淀粉酶。偶尔，一些小颗粒对两种分泌蛋白都显示双重标记。还存在许多含有胰岛素和/或淀粉酶的噬分泌体和自噬体。应用特异性探针进行的原位杂交证实了这些细胞中胰岛素和淀粉酶mRNA的存在。由于已知胆管结扎会诱导胰岛素细胞增殖，目前的结果证实内分泌腺泡细胞确实在这种情况下出现，并且可能代表转分化过程中的中间步骤。
