Onaga T, Uchida M, Kimura M, Miyazaki M, Mineo H, Kato S, Zabielski R
Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1996 Nov;115(3):185-93. doi: 10.1016/s0742-8413(96)00078-3.
The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the regulation of exocrine and endocrine pancreas was investigated in conscious sheep. Intravenous infusions of PACAP-27 and PACAP-38 (1, 3, and 10 pmol/kg/min) for 10 min during phase II of the duodenal migrating myoelectric complex accelerated pancreatic protein and amylase outputs dose-dependently. The responses in enzyme secretion to both PACAPs at the highest doses were inhibited significantly by atropine infusion (14.4 nmol/kg/min). Vasoactive intestinal polypeptide (VIP) at 3 pmol/kg/min significantly accelerated protein but not amylase outputs, although the response to the highest dose was not significantly influenced by atropine. PACAP-27 and VIP increased pancreatic juice flow and bicarbonate output dose-dependently; however, the responses to the highest dose were not altered significantly by atropine. On the other hand, intravenous injection of PACAP-38 (100 pmol/kg) did not influence basal plasma concentration of insulin, glucagon, and glucose. Moreover, PACAP-38 (1-100 pmol/kg) altered neither pancreatic endocrine response to intravenous infusion of glucose (20 mumol/kg/min) not that to n-butyric acid (33 mumol/kg/min). These results suggest that PACAP contributes to the regulation of exocrine secretion of the ovine pancreas but not to endocrine secretion. PACAP appears to accelerate pancreatic enzyme secretion mostly via the cholinergic nerves.
在清醒绵羊中研究了垂体腺苷酸环化酶激活多肽（PACAP）在外分泌和内分泌胰腺调节中的作用。在十二指肠移行性肌电复合波的II期，静脉输注PACAP - 27和PACAP - 38（1、3和10 pmol/kg/min）10分钟，剂量依赖性地加速了胰腺蛋白质和淀粉酶的分泌。阿托品输注（14.4 nmol/kg/min）显著抑制了两种PACAP在最高剂量时对酶分泌的反应。血管活性肠肽（VIP）以3 pmol/kg/min的剂量显著加速了蛋白质分泌，但未加速淀粉酶分泌，尽管对最高剂量的反应不受阿托品的显著影响。PACAP - 27和VIP剂量依赖性地增加了胰液流量和碳酸氢盐分泌；然而，阿托品对最高剂量的反应没有显著改变。另一方面，静脉注射PACAP - 38（100 pmol/kg）不影响胰岛素、胰高血糖素和葡萄糖的基础血浆浓度。此外，PACAP - 38（1 - 100 pmol/kg）既不改变胰腺对静脉输注葡萄糖（20 μmol/kg/min）的内分泌反应，也不改变对正丁酸（33 μmol/kg/min）的内分泌反应。这些结果表明，PACAP有助于调节绵羊胰腺的外分泌，但不参与内分泌调节。PACAP似乎主要通过胆碱能神经加速胰腺酶的分泌。
