使用组合文库和电喷雾电离-傅里叶变换离子回旋共振质谱法评估酶底物特异性。
Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.
作者信息
Wigger M, Nawrocki J P, Watson C H, Eyler J R, Benner S A
机构信息
Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
出版信息
Rapid Commun Mass Spectrom. 1997;11(16):1749-52. doi: 10.1002/(SICI)1097-0231(19971030)11:16<1749::AID-RCM91>3.0.CO;2-G.
A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.
已经进行了一项模型实验，该实验使用组合文库结合电喷雾电离-傅里叶变换离子回旋共振（ESI-FTICR）质谱，通过酶对底物文库进行“在线”筛选。监测了亲电底物1-氯-2,4-二硝基苯与H-γ-Glu-Cys-Xxx-OH文库的组分之间由谷胱甘肽-S-转移酶催化的反应。该实验表明了通过ESI-FTICR质谱对底物文库进行“二维”筛选的可行性。
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