Hofstadler S A, Severs J C, Smith R D, Swanek F D, Ewing A G
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Rapid Commun Mass Spectrom. 1996;10(8):919-22. doi: 10.1002/(SICI)1097-0231(19960610)10:8<919::AID-RCM597>3.0.CO;2-8.
Recent results with on-line capillary electrophoresis (CE) electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry suggest that CE/ESI-FTICR can provide a powerful technique for micro-sample analyses owing to the inherent sensitivity of the technique and the enhanced information content derived from high-performance mass measurements. Using micro-sampling methods and ion accumulation techniques based on quadrupolar excitation, we demonstrate that adequate sensitivity exists to characterize the hemoglobin from a single human erythrocyte (approximately 450 amol). In these studies mass spectra with average mass resolution in excess of 45 000 (FWHM) were obtained for both the alpha- and beta chain of hemoglobin following in-column lysing of a single erythrocyte.
近期在线毛细管电泳（CE）电喷雾电离（ESI）傅里叶变换离子回旋共振（FTICR）质谱分析的结果表明，由于该技术固有的灵敏度以及高性能质量测量所带来的信息含量增加，CE/ESI-FTICR可为微量样品分析提供强大的技术手段。利用基于四极激发的微量进样方法和离子积累技术，我们证明了存在足够的灵敏度来表征单个人类红细胞（约450阿托摩尔）中的血红蛋白。在这些研究中，对单个红细胞进行柱内裂解后，获得了血红蛋白α链和β链的平均质量分辨率超过45000（半高宽）的质谱图。
