Kobayashi A, Tizard I R, Woo P T
Am J Trop Med Hyg. 1976 May;25(3):401-6. doi: 10.4269/ajtmh.1976.25.401.
It was postulated that the anemia commonly seen in mammalian trypanosomiasis, and specifically in Trypanosoma congolense-infected calves, was of immunological origin. Specifically, we postulated that trypanosome antigen-antibody-complement complexes, deposited on the surface of erythrocytes of infected calves, resulted in their immune elimination leading to clinical anemia. This hypothesis was tested experimentally. Immunoglobulins bound to the erythrocytes of 13 infected calves were detected by a direct antiglobulin test from 7 to 10 days post infection. The reaction was strongest between 3 and 9 weeks and remained inconsistently positive until the calves were killed by euthanasia 15 to 18 weeks after infection. Erythrocytes reacting positively in this test were then lysed and immunoglobulins were eluted from the washed stromata by means of the low pH buffer. Sixteen out of 74 eluates prepared in this way and concentrated, contained IgM and IgG. Antibody activity of these eluates against T. congolense was demonstrated by means of the complement fixation test, the indirect hemagglutination test, and the indirect antiglobulin test. It is considered that the original hypothesis has been essentially proven.
据推测，哺乳动物锥虫病中常见的贫血，尤其是在感染刚果锥虫的犊牛中出现的贫血，是由免疫原因引起的。具体而言，我们推测锥虫抗原 - 抗体 - 补体复合物沉积在感染犊牛的红细胞表面，导致其被免疫清除，进而引发临床贫血。该假设通过实验进行了验证。在感染后7至10天，通过直接抗球蛋白试验检测了13头感染犊牛红细胞上结合的免疫球蛋白。反应在3至9周时最强，并且在感染后15至18周犊牛被安乐死之前一直呈不一致的阳性。然后将在此试验中呈阳性反应的红细胞裂解，并通过低pH缓冲液从洗涤后的基质中洗脱免疫球蛋白。以这种方式制备并浓缩的74份洗脱液中，有16份含有IgM和IgG。通过补体结合试验、间接血凝试验和间接抗球蛋白试验证明了这些洗脱液对刚果锥虫的抗体活性。可以认为最初的假设已基本得到证实。
