Gaspar C, Calvente M J, Fernández C, Fereres J
Servicio de Medicina Preventiva, Hospital Universitarío San Carlos, Madrid.
Enferm Infecc Microbiol Clin. 1997 May;15(5):250-4.
Microbiological standard for air in plenum ventilated operating theatres are not defined. The aim of this study was to analyze the microbiological contamination of air in HUSC theatres and to establish standards of reference.
408 air samples (80 l/sample) were taken from air intake and center of the theatres in three surgical areas, throughout five consecutive years (1991-1995). RCS air sampler was used. Media of microbiological counts were compared using the ANOVA and the Kruskall-Wallis tests. Microbiological standards were established based on upper limit of confidence interval of counts, when the degree of contamination was similar in two consecutive years.
A falling trend of microbiological counts was observed, with statistical significance between counts found in 1992 and those found in 1994 and 1995 (p < 0.01). There was no difference between results of the three surgical areas, but there was a significant difference between counts from air intake and center of theatres, regardless the year or the surgical area (p < 0.01). During this time a significant decrease in the percentage of samples with presence of fungi (p = 0.001) was produced. The results of years 1994 and 1995 were taken for establish the microbiological standards.
This control program allowed to evaluate and improve the hygienic conditions in the operating theatres and to establish a microbiological standards of reference for plenum ventilated theatres.
层流通风手术室的空气微生物标准尚未明确。本研究旨在分析哈斯特大学附属中心医院手术室空气的微生物污染情况并建立参考标准。
在连续五年（1991 - 1995年）期间，从三个手术区域的手术室进气口和中心采集了408份空气样本（80升/样本）。使用了RCS空气采样器。采用方差分析和克鲁斯卡尔 - 沃利斯检验对微生物计数培养基进行比较。当连续两年污染程度相似时，根据计数置信区间的上限建立微生物标准。
观察到微生物计数呈下降趋势，1992年的计数与1994年和1995年的计数之间具有统计学意义（p < 0.01）。三个手术区域的结果之间没有差异，但无论年份或手术区域如何，进气口和手术室中心的计数之间存在显著差异（p < 0.01）。在此期间，有真菌存在的样本百分比显著下降（p = 0.001）。采用1994年和1995年的结果来建立微生物标准。
该控制程序有助于评估和改善手术室的卫生状况，并为层流通风手术室建立微生物参考标准。
