Braselton W E, Stuart K J, Mullaney T P, Herdt T H
Animal Health Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing 48824, USA.
J Vet Diagn Invest. 1997 Oct;9(4):395-400. doi: 10.1177/104063879700900409.
Liver copper concentration is generally considered the best measure of copper nutritional status in cattle. Ultrasonic nebulization in conjunction with inductively coupled plasma-atomic emission spectroscopy (ICP-AES) was investigated as a method to provide adequate sensitivity to allow accurate simultaneous measurement of copper and 14 additional elements from needle biopsy samples. The element concentration frequency distribution profile of 12 elements routinely present in liver was compared to profiles of the elements in fat, muscle, vena cava, kidney, and clotted blood. The profiles could be used to confirm the authenticity of the liver biopsy sample. Element concentrations in biopsy samples taken in triplicate from the five lobes of a bovine liver were compared to those from triplicate wedge sections taken adjacent to the biopsies and analyzed by conventional ICP-AES. Precision between biopsies was equal to or better than precision between wedge samples. Some element concentrations determined by the biopsy procedure differed statistically from those determined by the wedge procedure, but differences were not sufficient to influence clinical interpretation of data.
肝脏铜浓度通常被认为是衡量牛铜营养状况的最佳指标。研究了将超声雾化与电感耦合等离子体原子发射光谱法（ICP-AES）相结合的方法，以提供足够的灵敏度，从而能够准确地同时测量来自针吸活检样本中的铜和其他14种元素。将肝脏中常规存在的12种元素的元素浓度频率分布曲线与脂肪、肌肉、腔静脉、肾脏和凝血中的元素曲线进行了比较。这些曲线可用于确认肝脏活检样本的真实性。将取自牛肝脏五个叶的活检样本一式三份中的元素浓度与取自活检相邻部位的一式三份楔形切片中的元素浓度进行比较，并通过传统的ICP-AES进行分析。活检之间的精密度等于或优于楔形样本之间的精密度。通过活检程序测定的一些元素浓度与通过楔形程序测定的元素浓度在统计学上存在差异，但差异不足以影响数据的临床解释。
