Chary-Valckenaere I, Guillemin F, Pourel J, Schiele F, Heller R, Jaulhac B
Department of Rheumatology, URA CNRS 1288, Centre Hospitalier Universitaire de Nancy, France.
Br J Rheumatol. 1997 Sep;36(9):945-9. doi: 10.1093/rheumatology/36.9.945.
This study investigates the seroreactivity to Borrelia burgdorferi antigens of patients suffering from rheumatoid arthritis (RA) for < 5 yr. Subjects were matched with controls for age, sex and area of residence in order to minimize the risk of differential exposure to B. burgdorferi. A total of 57 pairs were tested by immunofluorescence assay (IFA) and Western blotting. Only two RA patients showed positive IFA results, and another was positive by Western blot analysis. No significant difference in Lyme seropositivity was detected between cases and controls using either serological test. Furthermore, no significant difference in antibody response was observed to specific or non-specific proteins (e.g. Osp C, 93 kDa protein, flagellin, heat shock proteins). These results do not support the previous suggestions that B. burgdorferi may be involved in the aetiology of RA, or that RA favours the production of antibodies that cross-react with B. burgdorferi proteins.
本研究调查了病程小于5年的类风湿关节炎（RA）患者对伯氏疏螺旋体抗原的血清反应性。研究对象在年龄、性别和居住地区方面与对照组进行了匹配，以尽量降低伯氏疏螺旋体暴露差异的风险。总共57对研究对象通过免疫荧光测定法（IFA）和蛋白质印迹法进行了检测。只有两名RA患者IFA结果呈阳性，另有一名患者蛋白质印迹分析呈阳性。使用任何一种血清学检测方法，病例组和对照组之间的莱姆病血清阳性率均未检测到显著差异。此外，针对特异性或非特异性蛋白质（如Osp C、93 kDa蛋白质、鞭毛蛋白、热休克蛋白）的抗体反应也未观察到显著差异。这些结果不支持先前的观点，即伯氏疏螺旋体可能参与RA的病因学，或者RA有利于产生与伯氏疏螺旋体蛋白发生交叉反应的抗体。
