Jaulhac B, Heller R, Limbach F X, Hansmann Y, Lipsker D, Monteil H, Sibilia J, Piémont Y
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur and Hôpitaux Universitaires de Strasbourg, Hôpitaux Universitaires de Strasbourg, 6700 Strasbourg, France.
J Clin Microbiol. 2000 May;38(5):1895-900. doi: 10.1128/JCM.38.5.1895-1900.2000.
Since Lyme arthritis was first described in the United States, it has now been reported in many countries of Europe. However, very few strains of the causative bacterium, Borrelia burgdorferi, have been isolated from synovial samples. For this reason, different molecular direct typing methods were developed recently to assess which species could be involved in Lyme arthritis in Europe. We developed a simple oligonucleotide typing method with PCR fragments from the flagellin gene of B. burgdorferi sensu lato, which is able to differentiate seven different Borrelia species. Among 10 consecutive PCR-positive patients with Lyme arthritis from the northeastern France, two species were identified in synovial samples: B. burgdorferi sensu stricto in 9 cases and B. garinii in 1 case. Conversely, all B. burgdorferi sensu lato species detected in 10 consecutive PCR-positive biopsies from a second set of patients with erythema migrans from the same geographical area were identified as either B. afzelii or B. garinii (P < 0.001). These results indicate that B. burgdorferi sensu stricto is the principal but not the only Borrelia species involved in Lyme arthritis in northeastern France.
自莱姆关节炎在美国首次被描述以来，如今在欧洲许多国家都有相关报道。然而，从滑膜样本中分离出的致病细菌——伯氏疏螺旋体的菌株非常少。因此，最近开发了不同的分子直接分型方法，以评估欧洲哪些物种可能与莱姆关节炎有关。我们开发了一种简单的寡核苷酸分型方法，利用伯氏疏螺旋体复合群鞭毛蛋白基因的PCR片段，该方法能够区分七种不同的疏螺旋体物种。在法国东北部连续10例PCR检测呈阳性的莱姆关节炎患者中，滑膜样本中鉴定出两种物种：9例为狭义伯氏疏螺旋体，1例为伽氏疏螺旋体。相反，在来自同一地理区域的另一组连续10例游走性红斑患者的PCR检测呈阳性的活检样本中检测到的所有伯氏疏螺旋体复合群物种均被鉴定为阿氏疏螺旋体或伽氏疏螺旋体（P < 0.001）。这些结果表明，狭义伯氏疏螺旋体是法国东北部莱姆关节炎的主要但并非唯一的疏螺旋体物种。
