Miyoshi E, Uozumi N, Noda K, Hayashi N, Hori M, Taniguchi N
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
Int J Cancer. 1997 Sep 17;72(6):1117-21. doi: 10.1002/(sici)1097-0215(19970917)72:6<1117::aid-ijc29>3.0.co;2-#.
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-6 fucosyltransferase (alpha1-6FucT) catalyzes the transfer of a fucosyl residue from GDP-fucose to the asparagine-linked GlcNAc residue of complex N-glycans via alpha1-6 linkage. These oligosaccharide structures are essential for the attachment of polysialic acid to the neural-cell-adhesion molecule, and its levels are useful for the differential diagnosis of hepatocellular carcinomas with respect to the microheterogeneity of alpha-fetoprotein. We have been successful in the purification and cDNA cloning of alpha1-6FucT from porcine brain and from a human gastric-cancer cell line. In the present study, mRNA expression of alpha1-6FucT in various rat tissues and human cancer cell lines was examined, along with the expression of alpha1-6FucT mRNA and the induction by treatment with several cytokines. Northern-blot analysis indicated high expression levels of alpha1-6FucT in brain and gastrointestinal-tract tissues of normal rats, as well as for a number of lung-cancer, gastric-cancer and colon-cancer cell lines. Although various cytokines did not induce alpha1-6FucT mRNA, differentiation of a tumor cell enhanced the mRNA by 2- to 3-fold. These results may provide new insight into studies on alpha1-6FucT in terms of carcinogenesis or differentiation.
GDP-L-岩藻糖：N-乙酰-β-D-葡糖胺α1-6岩藻糖基转移酶（α1-6FucT）催化岩藻糖基从GDP-岩藻糖通过α1-6连接转移至复合N-聚糖的天冬酰胺连接的GlcNAc残基上。这些寡糖结构对于多聚唾液酸与神经细胞黏附分子的连接至关重要，其水平对于肝细胞癌甲胎蛋白微异质性的鉴别诊断很有用。我们已成功从猪脑和人胃癌细胞系中纯化并克隆出α1-6FucT的cDNA。在本研究中，检测了α1-6FucT在各种大鼠组织和人癌细胞系中的mRNA表达，以及α1-6FucT mRNA的表达和几种细胞因子处理后的诱导情况。Northern印迹分析表明，正常大鼠的脑和胃肠道组织以及许多肺癌、胃癌和结肠癌细胞系中α1-6FucT表达水平较高。尽管各种细胞因子未诱导α1-6FucT mRNA，但肿瘤细胞的分化使该mRNA增加了2至3倍。这些结果可能为α1-6FucT在致癌作用或分化方面的研究提供新的见解。
