HpSS：一种用于幽门螺杆菌的新型银染色方法。
HpSS: a new silver staining method for Helicobacter pylori.
作者信息
Doglioni C, Turrin M, Macrì E, Chiarelli C, Germanà B, Barbareschi M
机构信息
Department of Pathology, City Hospital, Belluno, Italy.
出版信息
J Clin Pathol. 1997 Jun;50(6):461-4. doi: 10.1136/jcp.50.6.461.
AIMS
To verify whether the proposed new silver staining method compares favourably with other well established methods in the detection of Helicobacter pylori in gastric biopsies.
METHODS
One hundred and forty pairs of antral and fundic biopsies, routinely formalin fixed and paraffin wax embedded, from 70 consecutive unselected patients were stained with haematoxylin and eosin, modified Giemsa, and the proposed H pylori silver stain (HpSS). H pylori immunodetection was performed in the same material with a polyclonal antiserum against H pylori.
RESULTS
H pylori was detected in 89 biopsies from 48 patients with haematoxylin and eosin; in a further five biopsies (one antral and four fundic) with Giemsa stain, thereby identifying one more H pylori infected patient. The new silver staining method was positive in all the cases detected by these two methods and detected three extra infected patients (five more positive biopsies). Immunohistochemistry detected one more positive case (two positive biopsies) not identified by any of the other methods.
CONCLUSIONS
The HpSS method proposed is highly sensitive in detecting H pylori; it is simple and it compares well with other methods used routinely for evaluating gastric biopsies for H pylori.
目的
验证在胃活检组织中检测幽门螺杆菌时，所提出的新银染方法是否优于其他成熟方法。
方法
对70例连续入选的未经选择的患者的140对胃窦和胃底活检组织进行苏木精-伊红染色、改良吉姆萨染色以及所提出的幽门螺杆菌银染法（HpSS），这些组织均常规用福尔马林固定并石蜡包埋。同时用抗幽门螺杆菌多克隆抗血清对相同材料进行幽门螺杆菌免疫检测。
结果
苏木精-伊红染色在48例患者的89份活检组织中检测到幽门螺杆菌；吉姆萨染色在另外5份活检组织（1份胃窦和4份胃底）中检测到幽门螺杆菌，从而多识别出1例幽门螺杆菌感染患者。新银染方法在这两种方法检测出的所有病例中均呈阳性，并检测出另外3例感染患者（多5份阳性活检组织）。免疫组化检测到1例其他方法均未识别出的阳性病例（2份阳性活检组织）。
结论
所提出的HpSS方法在检测幽门螺杆菌方面高度敏感；它操作简单，与其他常用于评估胃活检组织中幽门螺杆菌的方法相比效果良好。
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