Frundzhian V G, Brovko L Iu, Karabasova M A, Ugarova N N
Prikl Biokhim Mikrobiol. 1997 Jul-Aug;33(4):455-60.
A bioluminescence assay for rapid (5-7 h) determination of susceptibility to antibiotics was applied to samples of septic blood and optimized. The method comprises hemolysis of blood, reduction of osmolarity by adding concentrated nutritive media, and further incubation of the samples in the presence or absence of therapeutic doses of the antibiotic examined. Growth of bacteria is estimated by the level of bacterial ATP in the sample, which is determined by a bioluminescence assay. Hemolysis and further incubation of samples in nutrition media reduced the concentration of nonbacterial ATP to a level that did not interfere with the determination of bacterial ATP. There was a positive correlation between the levels of resistance to antibiotics determined by the bioluminescence assay and standard plate counts.
一种用于快速（5 - 7小时）测定抗生素敏感性的生物发光测定法应用于败血症血液样本并进行了优化。该方法包括血液溶血、通过添加浓缩营养培养基降低渗透压，以及在有或没有所检测抗生素治疗剂量的情况下对样本进行进一步孵育。通过生物发光测定法测定样本中细菌ATP的水平来估计细菌的生长。样本在营养培养基中的溶血和进一步孵育将非细菌ATP的浓度降低到不干扰细菌ATP测定的水平。通过生物发光测定法测定的抗生素耐药水平与标准平板计数之间存在正相关。
