蓖麻籽胚乳中乙醛酸循环体膜和内质网膜多肽组成的相似性。

Similarities in the polypeptide composition of glyoxysomal and endoplasmic-reticulum membranes from castor-bean endosperm.

作者信息

Bowden L, Lord J M

出版信息

Biochem J. 1976 Feb 15;154(2):491-9. doi: 10.1042/bj1540491.

DOI:10.1042/bj1540491

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172729/

Abstract

Microsomal fractions, glyoxysomes and mitochondria were isolated from homogenates of germinating castor-bean (Ricinus communis) endosperm by sucrose-density-gradient centrifugation. Washed membrane preparations from these cellular fractions were examined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. At corresponding developmental stages the endoplasmic-reticulum and glyoxysomal membranes were strikingly similar in polypeptide composition, at least 16 polypeptides being present in membranes isolated from 3-day-old tissue. Supplying [35S]methionine to intact endosperm tissue resulted in the labelling of all membrane polypeptides, the specific radioactivity in the endoplasmic reticulum being greater than for equivalent polypeptides of the glyoxysomal membrane. Washing these membranes with sodium deoxycholate solution extensively solubilized protein components, with the exception of a predominant polypeptide of mol.wt. 55000. Mitochondrial membrane preparations differed from those of the endoplasmic reticulum and glyoxysomes in polypeptide molecular-weight distribution and the [35S]methionine-labelling pattern. The similarity in polypeptide composition between endoplasmic-reticulum and glyoxysomal membranes is discussed in relation to glyoxysome biogenesis.

摘要

通过蔗糖密度梯度离心法，从萌发的蓖麻（Ricinus communis）胚乳匀浆中分离出微粒体组分、乙醛酸循环体和线粒体。对这些细胞组分的洗涤膜制剂进行十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析。在相应的发育阶段，内质网和乙醛酸循环体膜在多肽组成上惊人地相似，从3日龄组织中分离出的膜中至少存在16种多肽。向完整的胚乳组织供应[35S]甲硫氨酸会导致所有膜多肽被标记，内质网中的比放射性高于乙醛酸循环体膜中相应多肽的比放射性。用脱氧胆酸钠溶液洗涤这些膜会大量溶解蛋白质成分，但分子量为55000的主要多肽除外。线粒体膜制剂在多肽分子量分布和[35S]甲硫氨酸标记模式上与内质网和乙醛酸循环体的不同。本文结合乙醛酸循环体的生物发生讨论了内质网和乙醛酸循环体膜在多肽组成上的相似性。

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本文引用的文献

1

Enzymes of phospholipid metabolism in the endoplasmic reticulum of castor bean endosperm.油桐胚乳内质网中磷脂代谢的酶。

Plant Physiol. 1973 Jul;52(1):50-3. doi: 10.1104/pp.52.1.50.

2

Characterization of deoxyribonucleic Acid species from castor bean endosperm: inability to detect a unique deoxyribonucleic Acid species associated with glyoxysomes.蓖麻籽胚乳中脱氧核糖核酸种类的表征：无法检测到与乙醛酸循环体相关的独特脱氧核糖核酸种类。

Plant Physiol. 1973 May;51(5):902-6. doi: 10.1104/pp.51.5.902.

3

Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

J Biol Chem. 1951 Nov;193(1):265-75.

4

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。

J Biol Chem. 1969 Aug 25;244(16):4406-12.

5

Similarity and limited multiplicity of membrane proteins from rough and smooth endoplasmic reticulum.来自糙面内质网和滑面内质网的膜蛋白的相似性及有限多样性。

Science. 1970 Dec 11;170(3963):1222-3. doi: 10.1126/science.170.3963.1222.

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Developmental studies on glyoxysomes in Ricinus endosperm.蓖麻胚乳中乙醛酸循环体的发育研究

J Cell Biol. 1970 Jan;44(1):94-102. doi: 10.1083/jcb.44.1.94.

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Localization of enzymes within microbodies.酶在微体中的定位。

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