Biochemistry Department, University of Missouri, Columbia, MO 65211.
Plant Physiol. 1981 Oct;68(4):845-50. doi: 10.1104/pp.68.4.845.
Chromatographic analysis of organelle-associated citrate synthase activity revealed distinct mitochondrial and glyoxysomal forms of the enzyme. The chromatographic elution patterns on hydroxylapatite, carboxymethylcellulose and DEAE-cellulose of citrate synthase from the endosperm of 4.5-day-old castor bean seedlings revealed significant differences for mitochondrial and glyoxysomal activities of the enzyme. The endoplasmic reticulum-associated citrate synthase activity eluted from DEAE-cellulose in a pattern that was identical to that of the glyoxysomal activity. The same kinds of organelle specific isozyme elution patterns were observed with young, developing seedlings. Gibberellic acid-treatment of young seedlings increased total recoverable citrate synthase activity from endosperm tissue but did not modify the organelle specific isozyme relationships.
细胞器相关柠檬酸合酶活性的色谱分析显示出该酶存在明显的线粒体和乙醛酸体两种形式。4.5 天大的蓖麻种子胚乳中柠檬酸合酶的羟磷灰石、羧甲基纤维素和 DEAE-纤维素的色谱洗脱模式显示出酶的线粒体和乙醛酸体活性有显著差异。与乙醛酸体活性相同的模式,内质网相关的柠檬酸合酶活性从 DEAE-纤维素中洗脱出来。在年轻的、发育中的幼苗中观察到了相同的细胞器特异性同工酶洗脱模式。赤霉素处理幼苗增加了胚乳组织中总可回收的柠檬酸合酶活性,但并未改变细胞器特异性同工酶的关系。