发育蓖麻幼苗胚乳中柠檬酸合酶的细胞器特异性同工酶。
Organelle-specific isozymes of citrate synthase in the endosperm of developing ricinus seedlings.
机构信息
Biochemistry Department, University of Missouri, Columbia, MO 65211.
出版信息
Plant Physiol. 1981 Oct;68(4):845-50. doi: 10.1104/pp.68.4.845.
Abstract
Chromatographic analysis of organelle-associated citrate synthase activity revealed distinct mitochondrial and glyoxysomal forms of the enzyme. The chromatographic elution patterns on hydroxylapatite, carboxymethylcellulose and DEAE-cellulose of citrate synthase from the endosperm of 4.5-day-old castor bean seedlings revealed significant differences for mitochondrial and glyoxysomal activities of the enzyme. The endoplasmic reticulum-associated citrate synthase activity eluted from DEAE-cellulose in a pattern that was identical to that of the glyoxysomal activity. The same kinds of organelle specific isozyme elution patterns were observed with young, developing seedlings. Gibberellic acid-treatment of young seedlings increased total recoverable citrate synthase activity from endosperm tissue but did not modify the organelle specific isozyme relationships.
摘要
细胞器相关柠檬酸合酶活性的色谱分析显示出该酶存在明显的线粒体和乙醛酸体两种形式。4.5 天大的蓖麻种子胚乳中柠檬酸合酶的羟磷灰石、羧甲基纤维素和 DEAE-纤维素的色谱洗脱模式显示出酶的线粒体和乙醛酸体活性有显著差异。与乙醛酸体活性相同的模式，内质网相关的柠檬酸合酶活性从 DEAE-纤维素中洗脱出来。在年轻的、发育中的幼苗中观察到了相同的细胞器特异性同工酶洗脱模式。赤霉素处理幼苗增加了胚乳组织中总可回收的柠檬酸合酶活性，但并未改变细胞器特异性同工酶的关系。
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本文引用的文献
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Effect of gibberellin a(3) on the endoplasmic reticulum and on the formation of glyoxysomes in the endosperm of germinating castor bean.赤霉素 A3 对萌发蓖麻胚乳中内质网和乙醛酸体形成的影响。
Plant Physiol. 1978 Sep;62(3):449-53. doi: 10.1104/pp.62.3.449.
2
Evidence that glyoxysomal malate synthase is segregated by the endoplasmic reticulum.证据表明乙醛酸循环体苹果酸合酶是通过内质网分隔的。
Plant Physiol. 1978 Feb;61(2):266-70. doi: 10.1104/pp.61.2.266.
3
Purification and comparative properties of microsomal and glyoxysomal malate synthase from castor bean endosperm.蓖麻籽胚乳微粒体和乙醛酸循环体苹果酸合酶的纯化及比较性质
Plant Physiol. 1978 Feb;61(2):259-65. doi: 10.1104/pp.61.2.259.
4
Role of the endoplasmic reticulum in glyoxysome formation in castor bean endosperm.内质网在蓖麻胚乳乙醛酸体形成中的作用。
Plant Physiol. 1976 Mar;57(3):406-9. doi: 10.1104/pp.57.3.406.
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The development of microbodies (glyoxysomes and leaf peroxisomes) in cotyledons of germinating watermelon seedlings.发芽西瓜幼苗子叶中微体（乙醛酸体和叶过氧化物体）的发育。
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6
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