Heuser L, Gregg R, Max M H
Am Surg. 1979 Dec;45(12):771-3.
To determine the possible role of 5-hydroxytryptamine (5-HT) in the formation of restraint ulcerations, we investigated the relationship among mucosal tissue levels of 5-HT, vagotomy, and restraint ulcerations. Three murine experimental groups and one control group were used to compare the ulcerogenic effects of restraint with and without prior vagotomy. Ulcers were graded as to frequency, size, and hemorrhage on a 0-4+ scale. 5HT was measured in the gastric mucosa of each animal. Restraint caused significant ulcerations. The mean tissue level of 5-HT was significantly higher in the group with marked ulcerations (p less than 0.001). Our data suggest an increased cellular production of 5-HT during the development of the restraint ulcers. Vagotomy protected the animals against restraint ulcerations without depleting the baseline levels of 5-HT. Previous experiments have shown that urine levels of 5-hydroxyindoleacetic acid (5-HIAA) increase during the restraint period. Thus, increased tissue and urine levels of 5-HT appear to coincide with the development of restraint stress ulcers in the rat. Accordingly, if the coincidence occurs in humans, the measurement of urinary levels of 5-HIAA may be an indirect means of determining those patients at high risk for the development of stress ulcers.
为了确定5-羟色胺（5-HT）在束缚性溃疡形成中的可能作用，我们研究了5-HT的黏膜组织水平、迷走神经切断术和束缚性溃疡之间的关系。使用三个小鼠实验组和一个对照组来比较有无预先迷走神经切断术时束缚的致溃疡作用。根据频率、大小和出血情况将溃疡按0-4+级进行分级。测定每只动物胃黏膜中的5HT。束缚会导致明显的溃疡。在有明显溃疡的组中，5-HT的平均组织水平显著更高（p<0.001）。我们的数据表明在束缚性溃疡发展过程中5-HT的细胞产生增加。迷走神经切断术可保护动物免受束缚性溃疡的影响，且不会消耗5-HT的基线水平。先前的实验表明在束缚期间5-羟吲哚乙酸（5-HIAA）的尿液水平会升高。因此，5-HT的组织和尿液水平升高似乎与大鼠束缚应激性溃疡的发展同时出现。相应地，如果这种同时出现的情况在人类中也存在，那么测定5-HIAA的尿液水平可能是确定那些有发生应激性溃疡高风险患者的一种间接方法。
