枯草芽孢杆菌168中丰原素操纵子的序列完成、鉴定及定义
Sequence completion, identification and definition of the fengycin operon in Bacillus subtilis 168.
作者信息
Tosato Valentina, Albertini Alessandra M, Zotti Michela, Sonda Sabrina, Bruschi Carlo V
机构信息
Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche,Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste,Italy.
Department of Genetics and Microbiology A. Buzzati Traverso, University of Pavia,27100 Pavia,Italy.
出版信息
Microbiology (Reading). 1997 Nov;143 ( Pt 11):3443-3450. doi: 10.1099/00221287-143-11-3443.
A 15 kb DNA fragment from the Bacillus subtilis chromosome between citB and ppsC has been sequenced, and new ORFs encoding putative enzymes involved in lipopolypeptide synthesis, which complete a partial operon previously reported, and a new set of enzymes responsible for lipid metabolism have been identified. From the analysis of DNA sequence homology of the fragment it was deduced that these new peptide synthetase genes are part of an operon for the biosynthesis of the fungicide fengycin.
对枯草芽孢杆菌染色体上位于citB和ppsC之间的一段15 kb DNA片段进行了测序，鉴定出了编码参与脂肽合成的假定酶的新开放阅读框（ORF），这些酶完善了先前报道的一个部分操纵子，还发现了一组负责脂质代谢的新酶。通过对该片段DNA序列同源性的分析推断，这些新的肽合成酶基因是杀菌剂丰原素生物合成操纵子的一部分。
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